Inflammation from Sleep Fragmentation Starts in the Periphery Rather than Brain in Male Mice.

Autor:	Nguyen VT; Western Kentucky University., Fields CJ; Western Kentucky University., Ashley NT; Western Kentucky University.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2023 Feb 13. Date of Electronic Publication: 2023 Feb 13.
DOI:	10.21203/rs.3.rs-2544592/v1
Abstrakt:	Obstructive sleep apnea is increasing worldwide, leading to disordered sleep patterns and inflammatory responses in brain and peripheral tissues that predispose individuals to chronic disease. Pro-inflammatory cytokines activate the inflammatory response and are normally regulated by glucocorticoids secreted from adrenal glands. However, the temporal dynamics of inflammatory responses and hypothalamic-pituitary-adrenal (HPA) axis activation in relation to acute sleep fragmentation (ASF) are undescribed. Male C57BL/6J mice were exposed to ASF or control conditions (no ASF) over specified intervals (1, 2, 6, and 24 h) and cytokine gene expression (IL-1beta, TNF-alpha) in brain and peripheral tissues as well as serum glucocorticoid and interleukin-6 (IL-6) concentration were assessed. The HPA axis was rapidly activated, leading to elevated serum corticosterone from 1-24 h of ASF compared with controls. This activation was followed by elevated serum IL-6 concentration from 6-24 h of ASF. The tissue to first exhibit increased pro-inflammatory gene expression from ASF was heart (1 h of ASF). In contrast, pro-inflammatory gene expression was suppressed in hypothalamus after 1 h of ASF, but elevated after 6 h. Because the HPA axis was activated throughout ASF, this suggests that brain, but not peripheral, pro-inflammatory responses were rapidly inhibited by glucocorticoid immunosuppression. Competing Interests: Competing Interests The authors declare that they have no known competing financial interests.
Databáze:	MEDLINE
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