A pillar/perfusion plate enhances cell growth, reproducibility, throughput, and user friendliness in dynamic 3D cell culture.

Autor:	Reddy Lekkala VK; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Kang SY; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Liu J; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Shrestha S; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Acharya P; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Joshi P; Bioprinting Laboratories Inc., Denton, Texas., Zolfaghar M; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Lee M; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Jamdagneya P; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Pagnis S; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Kundi A; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Kabbur S; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Kim UT; Department of Civil and Environmental Engineering, Cleveland State University, Cleveland, Ohio., Yang Y; Department of Biomedical Engineering, University of North Texas, Denton, Texas., Lee MY; Department of Biomedical Engineering, University of North Texas, Denton, Texas.; Bioprinting Laboratories Inc., Denton, Texas.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 Feb 19. Date of Electronic Publication: 2023 Feb 19.
DOI:	10.1101/2023.02.16.528892
Abstrakt:	Static three-dimensional (3D) cell culture has been demonstrated in ultralow attachment well plates, hanging droplet plates, and microtiter well plates with hydrogels or magnetic nanoparticles. Although it is simple, reproducible, and relatively inexpensive, thus potentially used for high-throughput screening, statically cultured 3D cells often suffer from the necrotic core due to limited nutrient and oxygen diffusion and waste removal and have limited in vivo -like tissue structure. Here, we overcome these challenges by developing a pillar/perfusion plate platform and demonstrating high-throughput, dynamic 3D cell culture. Cell spheroids have been loaded on the pillar plate with hydrogel by simple sandwiching and encapsulation and cultured dynamically in the perfusion plate on a digital rocker. Unlike traditional microfluidic devices, fast flow rates were maintained within perfusion wells, and the pillar plate could be separated from the perfusion plate for cell-based assays. It was compatible with common lab equipment and allowed cell culture, testing, staining, and imaging in situ . The pillar/perfusion plate enhanced cell growth by rapid diffusion, reproducibility, assay throughput, and user friendliness in dynamic 3D cell culture. Competing Interests: Competing interests M.Y.L. is the founder and president of Bioprinting Laboratories Inc., the company manufacturing and commercializing the pillar/perfusion plate platform.
Databáze:	MEDLINE
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