A simple and accessible CRISPR genome editing laboratory exercise using yeast.
| Autor: | Shortt C; College of Osteopathic Medicine of the Pacific Northwest, Western University of Health Sciences, Lebanon, Oregon, USA., Krippaehne E; College of Osteopathic Medicine of the Pacific Northwest, Western University of Health Sciences, Lebanon, Oregon, USA., Wasko BM; College of Osteopathic Medicine of the Pacific Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. |
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| Jazyk: | angličtina |
| Zdroj: | MicroPublication biology [MicroPubl Biol] 2023 Feb 06; Vol. 2023. Date of Electronic Publication: 2023 Feb 06 (Print Publication: 2023). |
| DOI: | 10.17912/micropub.biology.000699 |
| Abstrakt: | CRISPR is a revolutionary tool to engineer the genome. Herein, we describe a laboratory exercise designed to introduce students to CRISPR by editing the genome of yeast to disrupt the ADE2 gene, which results in yeast that form red colored colonies. The experiment was constructed to be performed in a single laboratory session and to be accessible to students and instructors without experience working with yeast. (Copyright: © 2023 by the authors.) |
| Databáze: | MEDLINE |
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