Domes and semi-capsules as model systems for infrared microspectroscopy of biological cells.

Autor: Solheim JH; Faculty of Science and Technology, Norwegian University of Life Sciences, 1430, Aas, Norway., Brandsrud MA; Faculty of Science and Technology, Norwegian University of Life Sciences, 1430, Aas, Norway. maren.brandsrud@nmbu.no., Kong B; Faculty of Science and Technology, Norwegian University of Life Sciences, 1430, Aas, Norway., Banyasz A; Laboratoire de Chimie de l'ENS de Lyon, French National Centre for Scientific Research (CNRS), 69364, Lyon, France., Borondics F; Synchrotron SOLEIL, L'Orme des Merisiers, Saint-Aubin-BP48, Gif-sur-Yvette CEDEX, France., Micouin G; Laboratoire de Chimie de l'ENS de Lyon, French National Centre for Scientific Research (CNRS), 69364, Lyon, France., Lossius S; Faculty of Science and Technology, Norwegian University of Life Sciences, 1430, Aas, Norway., Sulé-Suso J; School of Pharmacy and Bioengineering, Cancer Centre, University Hospitals of North Midlands, Keele University, Stoke on Trent, ST4 6QG, UK., Blümel R; Department of Physics, Wesleyan University, Middletown, CT, USA., Kohler A; Faculty of Science and Technology, Norwegian University of Life Sciences, 1430, Aas, Norway.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Feb 23; Vol. 13 (1), pp. 3165. Date of Electronic Publication: 2023 Feb 23.
DOI: 10.1038/s41598-023-30130-z
Abstrakt: It is well known that infrared microscopy of micrometer sized samples suffers from strong scattering distortions, attributed to Mie scattering. The state-of-the-art preprocessing technique for modelling and removing Mie scattering features from infrared absorbance spectra of biological samples is built on a meta model for perfect spheres. However, non-spherical cell shapes are the norm rather than the exception, and it is therefore highly relevant to evaluate the validity of this preprocessing technique for deformed spherical systems. Addressing these cases, we investigate both numerically and experimentally the absorbance spectra of 3D-printed individual domes, rows of up to five domes, two domes with varying distance, and semi-capsules of varying lengths as model systems of deformed individual cells and small cell clusters. We find that coupling effects between individual domes are small, corroborating previous related literature results for spheres. Further, we point out and illustrate with examples that, while optical reciprocity guarantees the same extinction efficiency for top vs. bottom illumination, a scatterer's internal field may be vastly different in these two situations. Finally, we demonstrate that the ME-EMSC model for preprocessing infrared spectra from spherical biological systems is valid also for deformed spherical systems.
(© 2023. The Author(s).)
Databáze: MEDLINE
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