A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution.

Autor:	Meissner GW; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Nern A; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Dorman Z; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., DePasquale GM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Forster K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Gibney T; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Hausenfluck JH; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., He Y; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Iyer NA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Jeter J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Johnson L; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Johnston RM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Lee K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Melton B; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Yarbrough B; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Zugates CT; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Clements J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Goina C; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Otsuna H; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Rokicki K; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Svirskas RR; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Aso Y; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Card GM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Dickson BJ; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Ehrhardt E; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Goldammer J; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Ito M; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Kainmueller D; Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Korff W; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Mais L; Max-Delbrueck-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany., Minegishi R; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Namiki S; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Rubin GM; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Sterne GR; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Wolff T; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States., Malkesman O; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Feb 23; Vol. 12. Date of Electronic Publication: 2023 Feb 23.
DOI:	10.7554/eLife.80660
Abstrakt:	Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here, we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 74,000 such adult central nervous systems. An anticipated use of this resource is to bridge the gap between neurons identified by electron or light microscopy. Identifying individual neurons that make up each GAL4 expression pattern improves the prediction of split-GAL4 combinations targeting particular neurons. To this end, we have made the images searchable on the NeuronBridge website. We demonstrate the potential of NeuronBridge to rapidly and effectively identify neuron matches based on morphology across imaging modalities and datasets. Competing Interests: GM, AN, ZD, GD, KF, TG, JH, YH, NI, JJ, LJ, RJ, KL, BM, BY, CZ, JC, CG, HO, KR, RS, YA, GC, BD, EE, JG, MI, DK, WK, LM, RM, SN, GR, GS, TW, OM No competing interests declared (© 2023, Meissner et al.)
Databáze:	MEDLINE
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