An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics.

Autor:	Kanthaswamy S; School of Mathematics and Natural Sciences, Arizona State University (ASU) at the West Campus, Glendale, AZ, USA.; California National Primate Research Center, University of California, Davis, CA, USA.; Evolutionary Biology Graduate Program, School of Life Sciences, Arizona State University, Tempe, AZ, USA., Brendel T; Eurofins Genomics Europe Applied Genomics GmbH, Ebersberg, Germany., Cancela L; IDENTITAS, Montevideo, Uruguay., Andrade de Oliveira DA; Laboratório de Genética, Escola de Veterinária da UFMG, Belo Horizonte, Brazil., Brenig B; Institute of Veterinary Medicine, University of Goettingen, Goettingen, Germany., Cons C; Facultad de Veterinaria, Laboratorio de Genética Bioquímica (LAGENBIO), Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain., Crespi JA; Facultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria (UNLP-CONICET LA PLATA), La Plata, Argentina., Dajbychová M; Genomia s.r.o., Plzeň, Czech Republic., Feldl A; Eurofins Genomics Europe Applied Genomics GmbH, Ebersberg, Germany., Itoh T; Maebashi Institute of Animal Science, Livestock Improvement Association of Japan (LIAJ), Maebashi, Japan., Landi V; Department of Veterinary Medicine, Animal Breeding and Genetics, University of Bari 'Aldo Moro', Valenzano, Italy., Martinez A; Department of Genetics, Faculty of Veterinary Sciences, University of Córdoba, Córdoba, Spain., Natonek-Wisniewska M; Department of Animal Molecular Biology, National Research Institute of Animal Production, Balice, Poland., Oldt RF; School of Mathematics and Natural Sciences, Arizona State University (ASU) at the West Campus, Glendale, AZ, USA.; California National Primate Research Center, University of California, Davis, CA, USA.; Evolutionary Biology Graduate Program, School of Life Sciences, Arizona State University, Tempe, AZ, USA., Radko A; Department of Animal Molecular Biology, National Research Institute of Animal Production, Balice, Poland., Ramírez O; Vetgenomics SL, Barcelona, Spain., Rodellar C; Facultad de Veterinaria, Laboratorio de Genética Bioquímica (LAGENBIO), Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Zaragoza, Spain., Ruiz-Girón M; Laboratorio de Biología Molecular y Genómica, Hispalis Biolab S.L.U., Seville, Spain., Schikorski D; Laboratoire LABOFARM-GENINDEXE, LOUDEAC Cedex, France., Turba ME; Genefast SRL, Forlì, Italy., Giovambatista G; Facultad de Ciencias Veterinarias UNLP, IGEVET - Instituto de Genética Veterinaria (UNLP-CONICET LA PLATA), La Plata, Argentina.
Jazyk:	angličtina
Zdroj:	Forensic sciences research [Forensic Sci Res] 2021 Jul 19; Vol. 7 (4), pp. 708-713. Date of Electronic Publication: 2021 Jul 19 (Print Publication: 2022).
DOI:	10.1080/20961790.2021.1886679
Abstrakt:	The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic short tandem repeat (STR) kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel's proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing. Competing Interests: No potential conflict of interest was reported by the authors. (© 2021 The Author(s). Published by Taylor & Francis Group on behalf of the Academy of Forensic Science.)
Databáze:	MEDLINE
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