Genomic insights of an alkaliphilic bacterium Halalkalibacter alkaliphilus sp. nov. isolated from an Indian Soda Lake.

Autor: Joshi A; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, 411021, Maharashtra, India. amaraja@nccs.res.in., Thite S; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, 411021, Maharashtra, India., Lodha T; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, 411021, Maharashtra, India., Joseph N; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, 411021, Maharashtra, India., Mengade P; National Centre for Microbial Resource (NCMR), National Centre for Cell Science, Sai Trinity Complex, Sus Road, Pashan, Pune, 411021, Maharashtra, India.
Jazyk: angličtina
Zdroj: Antonie van Leeuwenhoek [Antonie Van Leeuwenhoek] 2023 May; Vol. 116 (5), pp. 435-445. Date of Electronic Publication: 2023 Feb 22.
DOI: 10.1007/s10482-023-01816-1
Abstrakt: An alkaliphilic, Gram-stain-positive, non-motile, rod-shaped, and spore forming bacterial strain (MEB205 T ) was isolated from sediment sample collected from Lonar lake, India. The strain grew optimally at pH 10, NaCl concentration of 3.0% at 37 °C. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain MEB205 T belonged to the genus Halalkalibacter in the family Bacillaceae and shared the highest sequence similarity with H. okhensis Kh10-101 T (98.9%) followed by H. wakoensis N-1  T (98.7%). The assembled genome of strain MEB205 T has a total length of 4.8 Mb with a G + C content of 37.8%. The dDDH and OrthoANI values between strain MEB205 T and H. okhensis Kh10-101  T were 29.1% and 84.3%, respectively. Furthermore, the genome analysis revealed the presence of antiporter genes (nhaA and nhaD) and L-ectoine biosynthesis gene required for survival of the strain MEB205 T in alkaline-saline habitat. The major fatty acid was C 15:0 anteiso, C 16:0 and C 15:0 iso (> 10.0%). Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. meso-diaminopimelic acid was diagnostic diamino acid for cell wall peptidoglycan. Based on the polyphasic taxonomic studies, strain MEB205 T represent a novel species of the genus Halalkalibacter for which the name Halalkalibacter alkaliphilus sp. nov. (Type strain MEB205 T  = MCC 3863  T  = JCM 34004  T  = NCIMB 15406  T ) is proposed.
(© 2023. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
Databáze: MEDLINE
Externí odkaz: