A Membrane-Associated Light-Harvesting Model is Enabled by Functionalized Assemblies of Gene-Doubled TMV Proteins.

Autor: Dai J; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA., Wilhelm KB; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA., Bischoff AJ; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA., Pereira JH; Technology Division, Joint BioEnergy Institute, Emeryville, CA, 94720, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA., Dedeo MT; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA., García-Almedina DM; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA., Adams PD; Technology Division, Joint BioEnergy Institute, Emeryville, CA, 94720, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.; Department of Bioengineering, University of California, Berkeley, Berkeley, CA, 94720, USA., Groves JT; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA., Francis MB; Department of Chemistry, University of California, Berkeley, Berkeley, CA, 94720, USA.; Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.
Jazyk: angličtina
Zdroj: Small (Weinheim an der Bergstrasse, Germany) [Small] 2023 May; Vol. 19 (20), pp. e2207805. Date of Electronic Publication: 2023 Feb 21.
DOI: 10.1002/smll.202207805
Abstrakt: Photosynthetic light harvesting requires efficient energy transfer within dynamic networks of light-harvesting complexes embedded within phospholipid membranes. Artificial light-harvesting models are valuable tools for understanding the structural features underpinning energy absorption and transfer within chromophore arrays. Here, a method for attaching a protein-based light-harvesting model to a planar, fluid supported lipid bilayer (SLB) is developed.  The protein model consists of the tobacco mosaic viral capsid proteins that are gene-doubled to create a tandem dimer (dTMV). Assemblies of dTMV break the facial symmetry of the double disk to allow for differentiation between the disk faces. A single reactive lysine residue is incorporated into the dTMV assemblies for the site-selective attachment of chromophores for light absorption. On the opposing dTMV face, a cysteine residue is incorporated for the bioconjugation of a peptide containing a polyhistidine tag for association with SLBs. The dual-modified dTMV complexes show significant association with SLBs and exhibit mobility on the bilayer. The techniques used herein offer a new method for protein-surface attachment and provide a platform for evaluating excited state energy transfer events in a dynamic, fully synthetic artificial light-harvesting system.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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