An Observational Study of Genetic Diversity of HIV-1 vpu in Rapid Progressors in India.
| Autor: | Gupta P; Molecular Virology Laboratory, Department of Biotechnology, Jamia Millia Islamia (Central University), 110 025, New Delhi, India., Rai A; National Centre for Disease Control, Ministry of Health & Family Welfare, 22 - Sham Nath Marg, 110 054, Delhi, India., Hans C; Department of Microbiology, Dr. Ram Manohar Lohia Hospital, 110 001, New Delhi, India., Husain M; Molecular Virology Laboratory, Department of Biotechnology, Jamia Millia Islamia (Central University), 110 025, New Delhi, India. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Current HIV research [Curr HIV Res] 2023; Vol. 21 (2), pp. 99-108. |
| DOI: | 10.2174/1570162X21666230221152633 |
| Abstrakt: | Background: The genetic diversity in HIV-1 genes affects viral pathogenesis in HIV-1 positive patients. Accessory genes of HIV-1, including vpu , are reported to play a critical role in HIV pathogenesis and disease progression. Vpu has a crucial role in CD4 degradation and virus release. The sequence heterogeneity in the vpu gene may affect disease progression in patients, therefore, the current study was undertaken to identify the role of vpu in patients defined as rapid progressors. Objective: The objective of the study was to identify the viral determinants present on vpu that may be important in disease progression in rapid progressors. Methods: Blood samples were collected from 13 rapid progressors. DNA was isolated from PBMCs and vpu was amplified using nested PCR. Both strands of the gene were sequenced using an automated DNA Sequencer. The characterization and analysis of vpu was done using various bioinformatics tools. Results: The analysis revealed that all sequences had intact ORF and sequence heterogeneity was present across all sequences and distributed all over the gene. The synonymous substitutions, however, were higher than nonsynonymous substitutions. The phylogenetic tree analysis showed an evolutionary relationship with previously published Indian subtype C sequences. Comparatively, the cytoplasmic tail Conclusion: The study showed that due to the robust nature of the protein, the biological activity of the protein was intact and sequence heterogeneity may promote disease progression in the study population. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|