| Autor: |
Hasegawa H; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology.; School of Life Science and Technology, Tokyo Institute of Technology., Kanesaki Y; Research Institute of Green Science and Technology, Shizuoka University., Watanabe S; Department of Bioscience, Tokyo University of Agriculture., Tanaka K; Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology. |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of general and applied microbiology [J Gen Appl Microbiol] 2023 Dec 05; Vol. 69 (3), pp. 167-174. Date of Electronic Publication: 2023 Feb 17. |
| DOI: |
10.2323/jgam.2023.02.001 |
| Abstrakt: |
Certain mutations of the model cyanobacterium Synechococcus elongatus PCC 7942 during laboratory storage have resulted in some divergent phenotypes. One laboratory-stored strain (H1) shows a temperature-sensitive (ts) growth phenotype at 40 °C. Here, we investigated the reason for this temperature sensitivity. Whole genome sequencing of H1 identified a single nucleotide mutation in synpcc7942_R0040 encoding tRNA-Leu(CAA). The mutation decreases the length of the tRNA-Leu t-arm from 5 to 4 base pairs, and this explains the ts phenotype. Secondary mutations suppressing the ts phenotype were identified in synpcc7942_1640, which putatively encodes a NYN domain-containing protein (nynA). The NYN domain is thought to be involved in tRNA/rRNA degradation. Thus, the structural stability of tRNA-Leu is critical for growth at 40 °C in Synechococcus elongatus PCC 7942. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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