CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems.

Autor: Ziemann M; Faculty of Biology, Institute of Biology III, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestr. 1, Freiburg, D-79104, Germany., Reimann V; Faculty of Biology, Institute of Biology III, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestr. 1, Freiburg, D-79104, Germany., Liang Y; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China.; Shandong Energy Institute, Qingdao, 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China., Shi Y; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China.; Shandong Energy Institute, Qingdao, 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China., Ma H; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China.; Shandong Energy Institute, Qingdao, 266101, China.; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Xie Y; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Li H; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China.; University of Chinese Academy of Sciences, Beijing, 100049, China., Zhu T; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China. zhutao@qibebt.ac.cn.; Shandong Energy Institute, Qingdao, 266101, China. zhutao@qibebt.ac.cn.; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China. zhutao@qibebt.ac.cn.; University of Chinese Academy of Sciences, Beijing, 100049, China. zhutao@qibebt.ac.cn., Lu X; Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT), Chinese Academy of Sciences, No.189 Songling Road, Qingdao, 266101, China. lvxf@qibebt.ac.cn.; Shandong Energy Institute, Qingdao, 266101, China. lvxf@qibebt.ac.cn.; Qingdao New Energy Shandong Laboratory, Qingdao, 266101, China. lvxf@qibebt.ac.cn.; University of Chinese Academy of Sciences, Beijing, 100049, China. lvxf@qibebt.ac.cn.; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. lvxf@qibebt.ac.cn., Hess WR; Faculty of Biology, Institute of Biology III, Genetics and Experimental Bioinformatics, University of Freiburg, Schänzlestr. 1, Freiburg, D-79104, Germany. wolfgang.hess@biologie.uni-freiburg.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Feb 18; Vol. 14 (1), pp. 924. Date of Electronic Publication: 2023 Feb 18.
DOI: 10.1038/s41467-023-36542-9
Abstrakt: Certain CRISPR-Cas elements integrate into Tn7-like transposons, forming CRISPR-associated transposon (CAST) systems. How the activity of these systems is controlled in situ has remained largely unknown. Here we characterize the MerR-type transcriptional regulator Alr3614 that is encoded by one of the CAST (AnCAST) system genes in the genome of cyanobacterium Anabaena sp. PCC 7120. We identify a number of Alr3614 homologs across cyanobacteria and suggest naming these regulators CvkR for Cas V-K repressors. Alr3614/CvkR is translated from leaderless mRNA and represses the AnCAST core modules cas12k and tnsB directly, and indirectly the abundance of the tracr-CRISPR RNA. We identify a widely conserved CvkR binding motif 5'-AnnACATnATGTnnT-3'. Crystal structure of CvkR at 1.6 Å resolution reveals that it comprises distinct dimerization and potential effector-binding domains and that it assembles into a homodimer, representing a discrete structural subfamily of MerR regulators. CvkR repressors are at the core of a widely conserved regulatory mechanism that controls type V-K CAST systems.
(© 2023. The Author(s).)
Databáze: MEDLINE
Externí odkaz: