Gold-binding peptide as a selective layer for electrochemical detection of SARS-CoV-2 antibodies.

Autor: Braz BA; Laboratory of Electrochemical Sensors (LabSensE), Department of Chemistry, Federal University of Paraná (UFPR), CEP 19032, CEP, 81531-980, Curitiba, PR, Brazil; Molecular Biology Laboratory, Graduate Program in Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), CEP, 81531-980, Curitiba, PR, Brazil., Hospinal-Santiani M; Molecular Biology Laboratory, Graduate Program in Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), CEP, 81531-980, Curitiba, PR, Brazil., Martins G; Laboratory of Electrochemical Sensors (LabSensE), Department of Chemistry, Federal University of Paraná (UFPR), CEP 19032, CEP, 81531-980, Curitiba, PR, Brazil., Gogola JL; Laboratory of Electrochemical Sensors (LabSensE), Department of Chemistry, Federal University of Paraná (UFPR), CEP 19032, CEP, 81531-980, Curitiba, PR, Brazil., Valenga MGP; Laboratory of Electrochemical Sensors (LabSensE), Department of Chemistry, Federal University of Paraná (UFPR), CEP 19032, CEP, 81531-980, Curitiba, PR, Brazil., Beirão BCB; Graduate Program in Microbiology, Parasitology, and Pathology, Federal University of Paraná (UFPR), CEP, 81531-980, Curitiba, PR, Brazil., Bergamini MF; Laboratory of Electrochemical Sensors (LabSensE), Department of Chemistry, Federal University of Paraná (UFPR), CEP 19032, CEP, 81531-980, Curitiba, PR, Brazil. Electronic address: bergamini@ufpr.br., Marcolino-Junior LH; Laboratory of Electrochemical Sensors (LabSensE), Department of Chemistry, Federal University of Paraná (UFPR), CEP 19032, CEP, 81531-980, Curitiba, PR, Brazil. Electronic address: luiz1berto@ufpr.br., Thomaz-Soccol V; Molecular Biology Laboratory, Graduate Program in Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), CEP, 81531-980, Curitiba, PR, Brazil., Soccol CR; Molecular Biology Laboratory, Graduate Program in Bioprocess Engineering and Biotechnology, Federal University of Paraná (UFPR), CEP, 81531-980, Curitiba, PR, Brazil.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2023 May 15; Vol. 257, pp. 124348. Date of Electronic Publication: 2023 Feb 11.
DOI: 10.1016/j.talanta.2023.124348
Abstrakt: Electrochemical immunosensors are excellent alternatives to prepare portable platforms used for rapid and inexpensive diagnostic of infectious diseases such as the recently emerged COVID-19. Incorporating synthetic peptides as selective recognition layers combined with nanomaterials such as gold nanoparticles (AuNPs) can significantly enhance the analytical performance of immunosensors. In the present study, an electrochemical immunosensor based on solid-binding peptide was built and evaluated towards SARS-CoV-2 Anti-S antibodies detection. The peptide used as recognition site has two important portions: one based on the viral receptor binding domain (RBD), capable of recognizing antibodies of the spike protein (Anti-S), and another suitable for interacting with gold nanoparticles. Gold-binding peptide (Pept/AuNP) dispersion was used directly to modify a screen-printed carbon electrode (SPE). The voltammetric behavior of the [Fe(CN) 6 ] 3-/4- probe after every construction and detection step was recorded using cyclic voltammetry by assessing the stability of the Pept/AuNP as a recognition layer onto the electrode surface. Differential pulse voltammetry was used as a detection technique, and a linear working range from 75 ng mL -1 to 15 μg mL -1 was established, with 1.059 μA dec -1 of sensitivity and R 2  = 0.984. The response selectivity against SARS-CoV-2 Anti-S antibodies was investigated in presence of concomitant species. The immunosensor was used to detect SARS-CoV-2 Anti-spike protein (Anti-S) antibodies in human serum samples, successfully differentiating between negative and positive responses of samples at a 95% confidence level. Therefore, the gold-binding peptide is a promising tool to be applied as a selective layer for antibody detection.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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