Gene mutations in the PI3K/Akt signaling pathway were related to immune thrombocytopenia pathogenesis.

Autor:	Ruan JS; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China., Sun RJ; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.; Department of Rheumatology, Peking Union Medical College Hospital, Clinical Immunology Center, Beijing, China., Wang JP; The Outpatient Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China., Sui XH; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China., Qu HT; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China., Yuan D; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China., Shan NN; Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Jazyk:	angličtina
Zdroj:	Medicine [Medicine (Baltimore)] 2023 Feb 17; Vol. 102 (7), pp. e32947.
DOI:	10.1097/MD.0000000000032947
Abstrakt:	Background: Immune thrombocytopenic (ITP) is an autoimmune bleeding disease with genetic susceptibility. Twenty newly diagnosed active primary ITP patients who had not been treated with glucocorticosteroids, immune globulin or immunosuppressants prior to sampling were enrolled in this study. Bone marrow blood mononuclear cells were used for whole exome sequencing to further elucidation the variant genes of ITP. Methods: High-molecular-weight genomic DNA was extracted from freshly frozen bone marrow blood mononuclear cells from 20 active ITP patients. Next, the samples were subjected to molecular genetic analysis by whole-exome sequencing, and the results were confirmed by Sanger sequencing. The signaling pathways and cellular processes associated with the mutated genes were identified with gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses. Results: The results showed that there were 3998 missense mutations involving 2269 genes in more than 10 individuals. Unique genetic variants including phosphatase and tensin homolog, insulin receptor, and coagulation factor C homology were the most associated with the pathogenesis of ITP. Functional analysis revealed these mutation genes mainly affect Phosphatidylinositol 3 kinase/serine/threonine kinase B signaling pathways (signal transduction) and platelet activation (immune system). Conclusion: Our finding further demonstrates the functional connections between these variant genes and ITP. Although the substantial mechanism and the impact of genetic variation are required further investigation, the application of next generation sequencing in ITP in this paper is a valuable method to reveal the genetic susceptibility. Competing Interests: The authors have no conflicts of interest to disclose. (Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.)
Databáze:	MEDLINE
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