STAM and Hrs interact sequentially with IFN-α Receptor to control spatiotemporal JAK-STAT endosomal activation.

Autor: Zanin N; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.; Centre National de la Recherche Scientifique (CNRS), Paris, France.; Namur Research Institute for Life Sciences (NARILIS), URBC, University of Namur, Namur, Belgium., Viaris de Lesegno C; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.; Centre National de la Recherche Scientifique (CNRS), Paris, France., Podkalicka J; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.; Centre National de la Recherche Scientifique (CNRS), Paris, France.; Laboratoire Physico-Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, Paris, France.; Laboratory of Cytobiochemistry, Faculty of Biotechnology, University of Wrocław, Wrocław, Poland., Meyer T; Department of Biology and Center for Cellular Nanoanalytics, University of Osnabruck, Osnabruck, Germany., Gonzalez Troncoso P; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.; Centre National de la Recherche Scientifique (CNRS), Paris, France., Bun P; Membrane Traffic in Healthy and Diseased Brain, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Université de Paris, Paris, France.; NeurImag Imaging Facility, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Université de Paris, Paris, France., Danglot L; Membrane Traffic in Healthy and Diseased Brain, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Université de Paris, Paris, France.; NeurImag Imaging Facility, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Université de Paris, Paris, France., Chmiest D; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France.; Centre National de la Recherche Scientifique (CNRS), Paris, France.; Department of Biochemistry, CIIL Biomedical Research Center, University of Lausanne, Epalinges, Switzerland., Urbé S; Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK., Piehler J; Department of Biology and Center for Cellular Nanoanalytics, University of Osnabruck, Osnabruck, Germany., Blouin CM; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France. cedric.blouin@curie.fr.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France. cedric.blouin@curie.fr.; Centre National de la Recherche Scientifique (CNRS), Paris, France. cedric.blouin@curie.fr., Lamaze C; Membrane Mechanics and Dynamics of Intracellular Signaling Laboratory, Institut Curie-Centre de Recherche, PSL Research University, Paris, France. christophe.lamaze@curie.fr.; Institut National de la Santé et de la Recherche Médicale (INSERM), Paris, France. christophe.lamaze@curie.fr.; Centre National de la Recherche Scientifique (CNRS), Paris, France. christophe.lamaze@curie.fr.
Jazyk: angličtina
Zdroj: Nature cell biology [Nat Cell Biol] 2023 Mar; Vol. 25 (3), pp. 425-438. Date of Electronic Publication: 2023 Feb 16.
DOI: 10.1038/s41556-022-01085-6
Abstrakt: Activation of the JAK-STAT pathway by type I interferons (IFNs) requires clathrin-dependent endocytosis of the IFN-α and -β receptor (IFNAR), indicating a role for endosomal sorting in this process. The molecular machinery that brings the selective activation of IFN-α/β-induced JAK-STAT signalling on endosomes remains unknown. Here we show that the constitutive association of STAM with IFNAR1 and TYK2 kinase at the plasma membrane prevents TYK2 activation by type I IFNs. IFN-α-stimulated IFNAR endocytosis delivers the STAM-IFNAR complex to early endosomes where it interacts with Hrs, thereby relieving TYK2 inhibition by STAM and triggering signalling of IFNAR at the endosome. In contrast, when stimulated by IFN-β, IFNAR signalling occurs independently of Hrs as IFNAR is sorted to a distinct endosomal subdomain. Our results identify the molecular machinery that controls the spatiotemporal activation of IFNAR by IFN-α and establish the central role of endosomal sorting in the differential regulation of JAK-STAT signalling by IFN-α and IFN-β.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
Externí odkaz: