Rapid in-situ growth of enzyme-mimicking Pd nanoparticles on TEMPO-oxidized nanocellulose for the efficient detection of ascorbic acid.

Autor: Dadigala R; Institute of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea., Bandi R; Institute of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea., Han SY; Institute of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea., Kwon GJ; Institute of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea; Kangwon Institute of Inclusion Technology, Kangwon National University, Chuncheon 24341, Republic of Korea., Lee SH; Institute of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea; Department of Forest Biomaterials Engineering, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea. Electronic address: lshyhk@kangwon.ac.kr.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2023 Apr 15; Vol. 234, pp. 123657. Date of Electronic Publication: 2023 Feb 14.
DOI: 10.1016/j.ijbiomac.2023.123657
Abstrakt: Rapid, efficient and green method of Pd nanoparticles (PdNPs) synthesis on TEMPO-oxidized cellulose nanofibril (TCNF) is demonstrated here. The nanohybrid (PdNPs/TCNF) exhibited peroxidase and oxidase-like activities evident by the oxidation of three chromogenic substrates. Enzyme kinetic studies using 3,3',5,5'-Tetramethylbenzidine (TMB) oxidation uncovered the excellent kinetic parameters (low K m and high V max ) and good specific activities of 215 U/g and 107 U/g for peroxidase and oxidase-like activities, respectively. A colorimetric assay for ascorbic acid (AA) detection is proposed based on its ability to reduce oxidized TMB to its colorless form. However, presence of nanozyme caused re-oxidation of TMB to its blue colored form within few minutes resulting in time limitation and inaccurate detection. Thanks to the film forming nature of TCNF; this limitation was overcome by employing PdNPs/TCNF film strips that can be easily removed before AA addition. The assay allowed AA detection in the linear range of 0.25-10 μM with a detection limit of 0.039 μM. The results of AA detection in commercial beverages and vitamin C tablets are matching with the specified values. Further the nanozyme exhibited high tolerance to pH (2-10) and temperature (up to 80 °C) and good recyclability for five cycles.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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