Repeated co-exposure to aflatoxin B 1 and aspartame disrupts the central nervous system homeostasis: Behavioral, biochemical, and molecular insights.

Autor: Souto NS; Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos, Universidade Federal de Santa Maria, Santa Maria, Brazil., Dassi M; Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos, Universidade Federal de Santa Maria, Santa Maria, Brazil., Braga ACM; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil., Rosa ÉVF; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil., Fighera MR; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil., Royes LFF; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil., Oliveira MS; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil., Sari MHM; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil., Furian AF; Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos, Universidade Federal de Santa Maria, Santa Maria, Brazil.; Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Jazyk: angličtina
Zdroj: Journal of food science [J Food Sci] 2023 Apr; Vol. 88 (4), pp. 1731-1742. Date of Electronic Publication: 2023 Feb 15.
DOI: 10.1111/1750-3841.16476
Abstrakt: Several studies demonstrated the toxicity of aspartame (ASP) and aflatoxin B 1 (AFB 1 ) in preclinical models. Although the majority of these reports assessed the toxic effects of each substance separately, their concomitant exposure and hazardous consequences are scarce. Importantly, the deleterious effects at the central nervous system caused by ASP and AFB 1 co-exposure are rarely addressed. We evaluated if concomitant exposure to AFB 1 and ASP would cause behavioral impairment and alteration in oxidative status of the brain in male rats. Animals received once a day for 14 days AFB 1 (250 µg/kg, intragastric gavage [i.g.]), ASP (75 mg/kg, i.g.), or both substances (association). On day 14, they were subjected to behavioral evaluation, and biochemical and molecular parameters of oxidative status were measured in the cerebral cortex and hippocampus. In the open field test, AFB 1 and combination treatments modified the motor, exploratory, and grooming behavior. In the splash test, all treatments caused a reduction in grooming time compared to the control group. An increase in thiobarbituric acid-reactive substances content induced by AFB 1 and combination treatments was observed. The antioxidant defenses (vitamin C, nonprotein sulfhydryl, and ferric reducing antioxidant power) were impaired in all groups compared to control. Regarding molecular evaluation, mitochondrial superoxide dismutase-2 immunoreactivity decreased after AFB 1 or ASP exposition in the hippocampus. Thus, co-exposure to ASP and AFB 1 was potentially more toxic because it aggravated behavioral impairments and oxidative status disbalance in comparison to the groups that received only ASP or AFB 1 . Therefore, our data suggest that those substances caused a disruption in brain homeostasis.
(© 2023 Institute of Food Technologists.)
Databáze: MEDLINE
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