Evaluation of N 6 -methyldeoxyadenosine antibody-based genomic profiling in eukaryotes.

Autor:	Debo BM; Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, Massachusetts 02115, USA., Mallory BJ; Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA., Stergachis AB; Department of Genome Sciences, University of Washington, Seattle, Washington 98195, USA; absterga@uw.edu.; Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, Washington 98195-7720, USA.; Brotman Baty Institute for Precision Medicine, Seattle, Washington 98195, USA.
Jazyk:	angličtina
Zdroj:	Genome research [Genome Res] 2023 Mar; Vol. 33 (3), pp. 427-434. Date of Electronic Publication: 2023 Feb 14.
DOI:	10.1101/gr.276696.122
Abstrakt:	Low-level DNA N 6 -methyldeoxyadenosine (DNA-m6A) has recently been reported across various eukaryotes. Although anti-m6A antibody-based approaches are commonly used to measure DNA-m6A levels, this approach is known to be confounded by DNA secondary structures, RNA contamination, and bacterial contamination. To evaluate for these confounding features, we introduce an approach for systematically validating the selectivity of antibody-based DNA-m6A methods and use a highly selective anti-DNA-m6A antibody to reexamine patterns of DNA-m6A in C. reinhardtii , A. thaliana , and D. melanogaster Our findings raise caution about the use of antibody-based methods for endogenous m6A quantification and mapping in eukaryotes. (© 2023 Debo et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu