Dynamics of annatto pigment synthesis and accumulation in seeds of Bixa orellana L. revealed by integrated chemical, anatomical, and RNA-Seq analyses.

Autor: Moreira VS; Instituto Federal de Educação Ciência E Tecnologia da Bahia, Euclides da Cunha, Bahia, 48500-000, Brazil., de Souza VC; Departamento de Ciências da Computação, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, 36036-900, Brazil., Soares VLF; Centro de Biotecnologia E Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, Bahia, 45662-900, Brazil., Sousa AO; Centro Multidisciplinar Do Campus Luís Eduardo Magalhães, Universidade Federal Do Oeste da Bahia, Luis Eduardo Magalhães, Bahia, 47850‑000, Brazil., de Nascimento KTS; Centro de Biotecnologia E Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, Bahia, 45662-900, Brazil., de Santana MR; Centro de Biotecnologia E Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, Bahia, 45662-900, Brazil., Rebouças TNH; Departamento de Fitotecnia E Zootecnia, Universidade Estadual Do Sudoeste da Bahia, Vitoria da Conquista, Bahia, 45083-900, Brazil., Leitão CAE; Departamento de Ciências Naturais, Universidade Estadual Do Sudoeste da Bahia, Vitoria da Conquista, Bahia, 45083-900, Brazil., Goliatt PVZC; Departamento de Ciências da Computação, Universidade Federal de Juiz de Fora, Juiz de Fora, Minas Gerais, 36036-900, Brazil., Faria DV; Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Vicosa, Minas Gerais, 36570-900, Brazil., Otoni WC; Departamento de Biologia Vegetal/BIOAGRO, Universidade Federal de Viçosa, Vicosa, Minas Gerais, 36570-900, Brazil., Costa MGC; Centro de Biotecnologia E Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilheus, Bahia, 45662-900, Brazil. marciogc.costa@gmail.com.
Jazyk: angličtina
Zdroj: Protoplasma [Protoplasma] 2023 Jul; Vol. 260 (4), pp. 1207-1219. Date of Electronic Publication: 2023 Feb 14.
DOI: 10.1007/s00709-023-01842-w
Abstrakt: Bixin is a commercially valuable apocarotenoid pigment found in the seed aril of Bixa orellana. The dynamics and regulation of its biosynthesis and accumulation during seed development remain largely unknown. Here, we combined chemical, anatomical, and transcriptomic data to provide stage-specific resolution of the cellular and molecular events occurring during B. orellana seed development. Seeds at five developmental stages (S1-S5) were used for analysis of bixin content and seed anatomy, and three of them (S1, S3, and S4) were selected for Illumina HiSeq sequencing. Bixin accumulated in large quantities in seeds compared with other tissues analyzed, particularly during the S2 stage, peaking at the S4 stage, and then decreasing slightly in the S5 stage. Anatomical analysis revealed that bixin accumulated in the large central vacuole of specialized cells, which were scattered throughout the developing mesotesta at the S2 stage, but enlarged progressively at later stages, until they occupied most of the parenchyma in the aril. A total of 13 million reads were generated and assembled into 73,381 protein-encoding contigs, from which 312 were identified as containing 1-deoxy-D-xylulose-5-phosphate/2-C-methyl-D-erythritol-4-phosphate (DOXP/MEP), carotenoid, and bixin pathways genes. Differential transcriptome expression analysis of these genes revealed that 50 of them were sequentially and differentially expressed through the seed developmental stages analyzed, including seven carotenoid cleavage dioxygenases, eight aldehyde dehydrogenases, and 22 methyltransferases. Taken together, these results show that bixin synthesis and accumulation in seeds of B. orellana are a developmentally regulated process involving the coordinated expression of DOXP/MEP, carotenoid, and bixin biosynthesis genes.
(© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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