Evidence for an early green/red photocycle that precedes the diversification of GAF domain photoreceptor cyanobacteriochromes.

Autor: Priyadarshini N; Faculty of Biology, Institute of Biology III, University of Freiburg, Schänzlestr. 1, 79104, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine, Albertstr. 19, 79104, Freiburg, Germany., Steube N; Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany., Wiens D; Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany., Narikawa R; Graduate School of Biological Sciences, Faculty of Science, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo, 192-0397, Japan., Wilde A; Faculty of Biology, Institute of Biology III, University of Freiburg, Schänzlestr. 1, 79104, Freiburg, Germany., Hochberg GKA; Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Str. 10, 35043, Marburg, Germany. georg.hochberg@mpi-marburg.mpg.de.; Faculty of Chemistry, University of Marburg, Hans-Meerwein-Str. 4, 35032, Marburg, Germany. georg.hochberg@mpi-marburg.mpg.de.; Center for Synthetic Microbiology (SYNMIKRO), Karl-von-Frisch-Str. 14, 35032, Marburg, Germany. georg.hochberg@mpi-marburg.mpg.de., Enomoto G; Faculty of Biology, Institute of Biology III, University of Freiburg, Schänzlestr. 1, 79104, Freiburg, Germany. genenomoto@gmail.com.; Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo, 182-8585, Japan. genenomoto@gmail.com.
Jazyk: angličtina
Zdroj: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2023 Jun; Vol. 22 (6), pp. 1415-1427. Date of Electronic Publication: 2023 Feb 14.
DOI: 10.1007/s43630-023-00387-4
Abstrakt: Phytochromes are linear tetrapyrrole-binding photoreceptors in eukaryotes and bacteria, primarily responding to red and far-red light signals reversibly. Among the GAF domain-based phytochrome superfamily, cyanobacteria-specific cyanobacteriochromes show various optical properties covering the entire visible region. It is unknown what physiological demands drove the evolution of cyanobacteriochromes in cyanobacteria. Here, we utilize ancestral sequence reconstruction and biochemical verification to show that the resurrected ancestral cyanobacteriochrome proteins reversibly respond to green and red light signals. pH titration analyses indicate that the deprotonation of the bound phycocyanobilin chromophore is crucial to perceive green light. The ancestral cyanobacteriochromes show only modest thermal reversion to the green light-absorbing form, suggesting that they evolved to sense the incident green/red light ratio. Many cyanobacteria can utilize green light for photosynthesis using phycobilisome light-harvesting complexes. The green/red sensing cyanobacteriochromes may have allowed better acclimation to changing light environments by rearranging the absorption capacity of the phycobilisome through chromatic acclimation.
(© 2023. The Author(s).)
Databáze: MEDLINE
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