Polyploid SNP Genotyping Using the MassARRAY System.

Autor:	da Costa Lima Moraes A; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Brazil., Sforça DA; Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, Brazil., Mancini MC; Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, Brazil., Vigna BBZ; Embrapa Pecuária Sudeste, Brazilian Agricultural Research Corporation, São Carlos, Brazil., de Souza AP; Department of Plant Biology, Biology Institute, University of Campinas (UNICAMP), Campinas, Brazil. anete@unicamp.br.; Center for Molecular Biology and Genetic Engineering (CBMEG), University of Campinas (UNICAMP), Campinas, Brazil. anete@unicamp.br.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2638, pp. 93-113.
DOI:	10.1007/978-1-0716-3024-2_7
Abstrakt:	Molecular marker discovery and genotyping are major challenges in polyploid breeding programs incorporating molecular biology tools. In this context, this work describes a method for single nucleotide polymorphism (SNP) genotyping in polyploid crops using matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry, the MassARRAY System. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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