SP22 sperm protein as a potential biomarker of fertility in humans: A preliminary study.

Autor: Rosa JL; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil. Electronic address: lima.rosa@unesp.br., Figueiredo TM; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., Barros JWF; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., Dos Santos DB; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., de Deus RS Junior; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., Borges CS; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., Kempinas WG; Institute of Biosciences, Department of Structural and Functional Biology, Laboratory of Reproductive and Developmental Biology and Toxicology, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil., Klinefelter GR; National Health and Environmental Effects Research Laboratory, Office of Research and Development, US Environmental Protection Agency, Research Triangle Park, NC, United States.
Jazyk: angličtina
Zdroj: Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2023 Apr; Vol. 117, pp. 108343. Date of Electronic Publication: 2023 Feb 11.
DOI: 10.1016/j.reprotox.2023.108343
Abstrakt: Infertility affects approximately 15% of couples of reproductive age, and 50% of the cases are directly related to men. The evaluation of male fertility is based on analyses of routine seminal parameters and the use of more advanced techniques can help identify fertility biomarkers. SP22 sperm protein is considered a biomarker in murine species since its concentration is highly correlated with sperm fertility. As the role of this protein as a biomarker is already well-established in other species, we hypothesized that this same correlation could apply to human. Thus, the present study aimed to investigate possible correlations between SP22 concentration and sperm parameters in fertile and infertile men. For this, a study was carried out on 21 volunteers' seminal samples who were grouped according to fertility as fertile (n = 10) or infertile (n = 11). Conventional and functional sperm analyses, membrane protein extraction, quantification and immunolocalization of SP22 were performed. The infertile volunteers showed an increase in the percentage of sperm with abnormalities in head morphology and a decrease in the percentage of sperm with intact plasma membrane and damaged acrosomal membrane. Serum concentration of the hormone SHBG was also decreased in infertile volunteers. The damage to the plasma membrane was positively correlated with the superoxide anion production. Although none of the functional parameters were correlated with SP22 concentration, type D sperm motility was negatively correlated and type A+B sperm motility was positively correlated. This preliminary study opens new paths in the characterization of SP22 as a non-invasive biomarker for predicting fertility/infertility.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Josiane de Lima Rosa reports financial support was provided by State of Sao Paulo Research Foundation.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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