Increased IFN-γ + and TNF-α + mucosal-associated invariant T cells in patients with aplastic anemia.

Autor: Chen X; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Zhang Y; Department of Hematology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China., Zhang Y; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Zhang Y; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Wang S; Department of Hematology, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China., Yu Z; Department of Hematology, First Affiliated Hospital, Jinan University, Guangzhou, China., Liu X; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Huang G; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Guo L; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Li X; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Zha X; Department of Clinical Laboratory, First Affiliated Hospital, Jinan University, Guangzhou, China., Li Y; Institute of Hematology, Medical College, Jinan University, Guangzhou, China., Li B; Institute of Hematology, Medical College, Jinan University, Guangzhou, China.
Jazyk: angličtina
Zdroj: Cytometry. Part B, Clinical cytometry [Cytometry B Clin Cytom] 2023 May; Vol. 104 (3), pp. 253-262. Date of Electronic Publication: 2023 Feb 13.
DOI: 10.1002/cyto.b.22115
Abstrakt: Background: Aplastic anemia (AA) is known as an autoimmune disease in which T cell activation is aberrant. It has been reported that unconventional T cells, mucosal-associated invariant T (MAIT) cells, play an important role in several autoimmune diseases, but it is unclear if they are involved in AA.
Methods: In this study, we for the first time analyzed the proportions, phenotypes, and cytokine properties of MAIT cells in AA by flow cytometry.
Results: We found that the percentage of circulating MAIT cells was generally higher for CD3 + , CD8 + , and CD8 - T cells in AA patients compared with healthy individuals. Moreover, the percentage of IL-18Rα-, NKG2D-, IFN-γ-, and TNF-α- positive MAIT cells was also significantly higher in AA patients. In addition, the percentage of IFN-γ + CD3 + or TNF-α + CD8 - MAIT cells had a significant negative correlation with the absolute neutrophil count.
Conclusions: We present the first observation of MAIT cells in patients with AA. MAIT cells are associated with a higher frequency of IFN-γ and TNF-α production and may contribute to the pathogenesis of AA.
(© 2023 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals LLC on behalf of International Clinical Cytometry Society.)
Databáze: MEDLINE
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