Robust biomarker discovery through multiplatform multiplex image analysis of breast cancer clinical cohorts.

Autor:	Eng J; Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR, 97239, USA.; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, 97239, USA., Bucher E; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, 97239, USA., Hu Z; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, 97239, USA., Sanders M; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA, USA.; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Chakravarthy B; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA, USA., Gonzalez P; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA, USA., Pietenpol JA; Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN, 37232, USA, USA.; Department of Biochemistry, Vanderbilt University, Nashville, TN, 37232, USA., Gibbs SL; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, 97239, USA., Sears RC; Department of Molecular and Medical Genetics, Oregon Health and Science University, Portland, OR, 97239, USA., Chin K; Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, 97239, USA.; Center for Early Detection Advanced Research, Oregon Health and Science University, Portland, OR, 97239, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2023 May 15. Date of Electronic Publication: 2023 May 15.
DOI:	10.1101/2023.01.31.525753
Abstrakt:	Spatial profiling of tissues promises to elucidate tumor-microenvironment interactions and enable development of spatial biomarkers to predict patient response to immunotherapy and other therapeutics. However, spatial biomarker discovery is often carried out on a single patient cohort or imaging technology, limiting statistical power and increasing the likelihood of technical artifacts. In order to analyze multiple patient cohorts profiled on different platforms, we developed methods for comparative data analysis from three disparate multiplex imaging technologies: 1) cyclic immunofluorescence data we generated from 102 breast cancer patients with clinical follow-up, in addition to publicly available 2) imaging mass cytometry and 3) multiplex ion-beam imaging data. We demonstrate similar single-cell phenotyping results across breast cancer patient cohorts imaged with these three technologies and identify cellular abundance and proximity-based biomarkers with prognostic value across platforms. In multiple platforms, we identified lymphocyte infiltration as independently associated with longer survival in triple negative and high-proliferation breast tumors. Then, a comparison of nine spatial analysis methods revealed robust spatial biomarkers. In estrogen receptor-positive disease, quiescent stromal cells close to tumor were more abundant in good prognosis tumors while tumor neighborhoods of mixed fibroblast phenotypes were enriched in poor prognosis tumors. In triple-negative breast cancer (TNBC), macrophage proximity to tumor and B cell proximity to T cells were greater in good prognosis tumors, while tumor neighborhoods of vimentin-positive fibroblasts were enriched in poor prognosis tumors. We also tested previously published spatial biomarkers in our ensemble cohort, reproducing the positive prognostic value of isolated lymphocytes and lymphocyte occupancy and failing to reproduce the prognostic value of tumor-immune mixing score in TNBC. In conclusion, we demonstrate assembly of larger clinical cohorts from diverse platforms to aid in prognostic spatial biomarker identification and validation.
Databáze:	MEDLINE
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