Protective Potentials of Alpha-Lipoic Acid against Ionizing Radiation-Induced Brain Damage in Rats.

Autor: Xu J; Department of Rehabilitation Medicine, 3201 Hospital, Hanzhong 723000, China., Alameri AA; University of Babylon, Babylon, Iraq., Zabibah RS; Medical Laboratory Technology Department, College of Medical Technology, The Islamic University, Najaf, Iraq., Gabr GA; Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia.; Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center, Giza, Egypt., Ramírez-Coronel AA; Azogues Campus Nursing Career, Health and Behavior Research Group (HBR), Psychometry and Ethology Laboratory, Catholic University of Cuenca, Ecuador.; Doctorate in Psychology, University of Palermo, Buenos Aires, Argentina.; Epidemiology and Biostatistics Research Group, CES University, Colombia., Bagheri H; Radiation Sciences Research Center (RSRC), AJA University of Medical Sciences, Tehran, Iran.; Radiation Biology Research Center, Iran University of Medical Sciences, Tehran, Iran., Abedi-Firouzjah R; Department of Medical Physics Radiobiology and Radiation Protection, School of Medicine, Babol University of Medical Sciences, Babol, Iran.
Jazyk: angličtina
Zdroj: Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2023 Feb 03; Vol. 2023, pp. 4999306. Date of Electronic Publication: 2023 Feb 03 (Print Publication: 2023).
DOI: 10.1155/2023/4999306
Abstrakt: Background: This study was aimed at determining the effects of alpha-lipoic acid on ionizing irradiation-induced oxidative damage and apoptosis in the brain of rats.
Methods: The animals were exposed to whole-brain X-radiation with a 15 Gy single dose in the absence or presence of alpha-lipoic acid (200 mg/kg body weight) pretreatment for one week. The rats were divided into four groups (5 rats in each group): vehicle control, alpha-lipoic acid alone (ALA), radiation alone (RAD), and radiation plus alpha-lipoic acid (RAD+ALA). In the next stage, malondialdehyde (MDA), nitric oxide, catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) in the brain tissue of the rats were measured. Furthermore, the Western blot analysis technique was performed to assess the NOX2, NOX4, and caspase-3 protein expression levels.
Results: Twenty-four hours after the irradiation, MDA and nitric oxide levels in the irradiated rats were significantly higher than those in the control group ( p < 0.001); however, the pretreatment with alpha-lipoic acid resulted in a significant reduction in these stress oxidative markers ( p < 0.05). Moreover, a significant decrease in CAT, SOD, and GPx levels was observed in the radiation group alone compared to the control group ( p < 0.01); in contrast, the activities of these antioxidant enzymes significantly increased in the radiation plus alpha-lipoic acid group in comparison to the radiation group alone ( p < 0.05). The results of Western blot analysis revealed that NOX2, NOX4, and caspase-3 protein expressions significantly elevated in the irradiated rats compared to the control group ( p < 0.001). The pretreatment with alpha-lipoic acid could significantly decrease the expression levels of NOX2, NOX4, and caspase-3 in comparison with the radiation group alone ( p < 0.05).
Conclusion: According to the obtained findings, it can be mentioned that the alpha-lipoic acid pretreatment could mitigate the ionizing irradiation-induced oxidative damage and apoptosis in the brain of the rats.
Competing Interests: The authors declare that there is no conflict of interest regarding the publication of this article.
(Copyright © 2023 Ji Xu et al.)
Databáze: MEDLINE
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