Evaluation of dual application of photodynamic therapy-PDT in Candida albicans.

Autor: Nunes IPF; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil., Crugeira PJL; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil; Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP:40110-100, Brazil., Sampaio FJP; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil., de Oliveira SCPS; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil; School of Dentistry, Ruy Barbosa Wyden University Center University (UniRuy Wyden), Av. Luís Viana Filho, 3172 - Paralela, Salvador - BA CEP: 41720-200, Brazil., Azevedo JM; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil; Department of Biology, Feira de Santana State University, Feira de Santa, BA 44036-900, Brazil., Santos CLO; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil., Soares LGP; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil., Samuel IDW; Organic Semiconductor Centre, SUPA, School of Physics & Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9SS, UK., Persheyev S; Organic Semiconductor Centre, SUPA, School of Physics & Astronomy, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9SS, UK., de Ameida PF; Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP:40110-100, Brazil., Pinheiro ALB; Center of Biophotonics, School of Dentistry, Federal University of Bahia - UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil. Electronic address: albp@ufba.br.
Jazyk: angličtina
Zdroj: Photodiagnosis and photodynamic therapy [Photodiagnosis Photodyn Ther] 2023 Jun; Vol. 42, pp. 103327. Date of Electronic Publication: 2023 Feb 10.
DOI: 10.1016/j.pdpdt.2023.103327
Abstrakt: This study aimed to evaluate, in vitro, the efficacy of photodynamic therapy - PDT using dimethyl methylene blue zinc chloride double salt (DMMB) and red LED light on planktonic cultures of Candida albicans. The tests were performed using the ATCC 90,028 strain grown at 37 °C for 24 h, according to a growth curve of C. albicans. The colonies were resuspended in sterile saline adjusted to a concentration of 2 × 10 8 cells / mL, with three experimental protocols being tested (Protocol 1, 2 and 3) with a fixed concentration of 750 ɳg/mL obtained through the IC 50 , and energy density 20 J/cm 2 . Protocol 1 was carried out using conventional PDT, Protocol 2 was applied double PDT in a single session, and Protocol 3 was applied double PDT in two sessions with a 24 h interval. The results showed logarithmic reductions of 3 (4.252575 ± 0.068526) and 4 logs (2.669533 ± 0.058592) of total fungal load in protocols 3 and 2 respectively in comparison to the Control (6.633547 ± 0.065384). Our results indicated that double application in a single session of PDT was the most effective approach for inhibiting the proliferation of Candida albicans (99.991% inhibition).
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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