Cytoskeleton Remodeling in Arabidopsis Stigmatic Cells Following Pollination.
| Autor: | Riglet L; The Sainsbury Laboratory, Bateman Street, CB2 1LR, University of Cambridge, Cambridge, UK., Fobis-Loisy I; Laboratoire Reproduction et Developpement des Plantes, Universite de Lyon, ENS de Lyon, CNRS, INRAE, Lyon, France. isabelle.fobis-loisy@ens-lyon.fr. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2604, pp. 215-225. |
| DOI: | 10.1007/978-1-0716-2867-6_16 |
| Abstrakt: | In plants, the first interaction that occurs between the male gametophytes (pollen grains) and the stigmatic epidermis of the female organ is crucial for successful reproduction. The stigma consists of a dome of flask-shaped cells specialized in pollen capture. In these stigmatic cells, the cytoskeleton network (cortical microtubules and actin microfilaments) actively responds to pollen contact and undergoes dynamic remodeling required for successful pollen acceptance to occur. Here, we have designed several microscopy mountings to monitor stigmatic cytoskeleton dynamics. These designs are based on the constraints linked to the tightly regulated pollen-stigma interaction and depend upon the experimental goal, either a static view or live-cell imaging. (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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