Carboxymethyl Cellulose-Based Polymers as Promising Matrices for Ficin Immobilization.

Autor: Sorokin AV; Biophysics and Biotechnology Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia.; Laboratory of Bioresource Potential of Coastal Area, Institute for Advanced Studies, Sevastopol State University, 33 Studencheskaya Street, 299053 Sevastopol, Russia.; Laboratory of Metagenomics and Food Biotechnologies, Voronezh State University of Engineering Technologies, 19 Revolutsii Avenue, 394036 Voronezh, Russia., Goncharova SS; Biophysics and Biotechnology Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia., Lavlinskaya MS; Biophysics and Biotechnology Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia.; Laboratory of Bioresource Potential of Coastal Area, Institute for Advanced Studies, Sevastopol State University, 33 Studencheskaya Street, 299053 Sevastopol, Russia.; Laboratory of Metagenomics and Food Biotechnologies, Voronezh State University of Engineering Technologies, 19 Revolutsii Avenue, 394036 Voronezh, Russia., Holyavka MG; Biophysics and Biotechnology Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia.; Laboratory of Bioresource Potential of Coastal Area, Institute for Advanced Studies, Sevastopol State University, 33 Studencheskaya Street, 299053 Sevastopol, Russia., Faizullin DA; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of the RAS, 2/31 Lobachevsky Street, 420111 Kazan, Russia., Kondratyev MS; Biophysics and Biotechnology Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia.; Laboratory of Structure and Dynamics of Biomolecular Systems, Institute of Cell Biophysics of the RAS, 3 Institutskaya Street, 142290 Pushchino, Russia., Kannykin SV; Material Science and Nanosystem Industry Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia., Zuev YF; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of the RAS, 2/31 Lobachevsky Street, 420111 Kazan, Russia., Artyukhov VG; Biophysics and Biotechnology Department, Voronezh State University, 1 Universitetskaya Square, 394018 Voronezh, Russia.
Jazyk: angličtina
Zdroj: Polymers [Polymers (Basel)] 2023 Jan 27; Vol. 15 (3). Date of Electronic Publication: 2023 Jan 27.
DOI: 10.3390/polym15030649
Abstrakt: The present work is devoted to research on the interaction between carboxymethyl cellulose sodium salt and its derivatives (graft copolymer of carboxymethyl cellulose sodium salt and N , N -dimethyl aminoethyl methacrylate) with cysteine protease (ficin). The interaction was studied by FTIR and by flexible molecular docking, which have shown the conjugates' formation with both matrices. The proteolytic activity assay performed with azocasein demonstrated that the specific activities of all immobilized ficin samples are higher in comparison with those of the native enzyme. This is due to the modulation of the conformation of ficin globule and of the enzyme active site by weak physical interactions involving catalytically valuable amino acids. The results obtained can extend the practical use of ficin in biomedicine and biotechnology.
Databáze: MEDLINE
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