| Autor: |
García-Fuentevilla L; Forest Sciences Institute (ICIFOR-INIA), CSIC, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain., Domínguez G; Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Spain., Martín-Sampedro R; Forest Sciences Institute (ICIFOR-INIA), CSIC, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain., Hernández M; Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Spain., Arias ME; Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Spain., Santos JI; General Services of Research SGIKER, University of the Basque Country (UPV/EHU), Edificio Joxe Mari Korta, Avda. Tolosa 72, 20018 San Sebastian, Spain., Ibarra D; Forest Sciences Institute (ICIFOR-INIA), CSIC, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain., Eugenio ME; Forest Sciences Institute (ICIFOR-INIA), CSIC, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain. |
| Abstrakt: |
Kraft lignin, a side-stream from the pulp and paper industry, can be modified by laccases for the synthesis of high added-value products. This work aims to study different laccase sources, including a bacterial laccase from Streptomyces ipomoeae (SiLA) and a fungal laccase from Myceliophthora thermophila (MtL), for kraft lignin polymerization. To study the influence of some variables in these processes, a central composite design (CCD) with two continuous variables (enzyme concentration and reaction time) and three levels for each variable was used. The prediction of the behavior of the output variables (phenolic content and molecular weight of lignins) were modelled by means of response surface methodology (RSM). Moreover, characterization of lignins was performed by Fourier-transform infrared (FTIR) spectroscopy and different nuclear magnetic resonance (NMR) spectroscopy techniques. In addition, antioxidant activity was also analyzed. Results showed that lignin polymerization (referring to polymerization as lower phenolic content and higher molecular weight) occurred by the action of both laccases. The enzyme concentration was the most influential variable in the lignin polymerization reaction within the range studied for SiLA laccase, while the most influential variable for MtL laccase was the reaction time. FTIR and NMR characterization analysis corroborated lignin polymerization results obtained from the RSM. |
