Bio-Prospecting of Crude Leaf Extracts from Thirteen Plants of Brazilian Cerrado Biome on Human Glioma Cell Lines.

Autor: Silva VAO; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil.; Department of Pathology, School of Medicine of the Federal University of Bahia, Salvador 40170-110, Brazil.; Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador 40296-710, Brazil., Rosa MN; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil., Gomes INF; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil., Vital PDS; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil., Alves ALV; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil., Evangelista AF; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil., Longato GB; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil.; Research Laboratory in Cellular and Molecular Biology of Tumors and Bioactive Compounds, San Francisco University, Bragança Paulista, São Paulo 12916-900, Brazil., Carloni AC; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil., Oliveira BG; Petroleomic and Forensic Laboratory, Chemistry Department, Federal University of Espírito Santo, Vitória 29075-910, Brazil., Pinto FE; Petroleomic and Forensic Laboratory, Chemistry Department, Federal University of Espírito Santo, Vitória 29075-910, Brazil., Romão W; Petroleomic and Forensic Laboratory, Chemistry Department, Federal University of Espírito Santo, Vitória 29075-910, Brazil., Rezende AR; Ituiutaba Unit, Department of Agrarian and Natural Sciences (DECAN), State University of Minas Gerais (UEMG), Divinopolis 38302-192, Brazil., Araújo AAC; Ituiutaba Unit, Department of Agrarian and Natural Sciences (DECAN), State University of Minas Gerais (UEMG), Divinopolis 38302-192, Brazil., Oliveira LSFM; Laboratory of Experimental Pathology, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil., Souza AAM; Laboratory of Experimental Pathology, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil., Oliveira SC; Laboratory of Experimental Pathology, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil., Ribeiro RIMA; Laboratory of Experimental Pathology, Federal University of São João del Rei-CCO/UFSJ, Divinópolis 35501-296, Brazil., Reis RM; Molecular Oncology Research Center, Barretos Cancer Hospital, São Paulo 14784-400, Brazil.; Life and Health Sciences Research Institute (ICVS), School of Health Sciences, University of Minho, 4710-057 Braga, Portugal.; ICVS/3B's-PT Government Associate Laboratory, 4710-057 Braga, Portugal.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2023 Feb 01; Vol. 28 (3). Date of Electronic Publication: 2023 Feb 01.
DOI: 10.3390/molecules28031394
Abstrakt: (1) Background: Malignant gliomas are aggressive tumors characterized by fast cellular growth and highly invasive properties. Despite all biological and clinical advances in therapy, the standard treatment remains essentially palliative. Therefore, searching for alternative therapies that minimize adverse symptoms and improve glioblastoma patients' outcomes is imperative. Natural products represent an essential source in the discovery of such new drugs. Plants from the cerrado biome have been receiving increased attention due to the presence of secondary metabolites with significant therapeutic potential. (2) Aim: This study provides data on the cytotoxic potential of 13 leaf extracts obtained from plants of 5 families (Anacardiaceae, Annonaceae, Fabaceae, Melastomataceae e Siparunaceae) found in the Brazilian cerrado biome on a panel of 5 glioma cell lines and one normal astrocyte. (3) Methods: The effect of crude extracts on cell viability was evaluated by MTS assay. Mass spectrometry (ESI FT-ICR MS) was performed to identify the secondary metabolites classes presented in the crude extracts and partitions. (4) Results: Our results revealed the cytotoxic potential of Melastomataceae species Miconia cuspidata, Miconia albicans, and Miconia chamissois . Additionally, comparing the four partitions obtained from M. chamissois crude extract indicates that the chloroform partition had the greatest cytotoxic activity against the glioma cell lines. The partitions also showed a mean IC 50 close to chemotherapy, temozolomide; nevertheless, lower toxicity against normal astrocytes. Analysis of secondary metabolites classes presented in these crude extracts and partitions indicates the presence of phenolic compounds. (5) Conclusions: These findings highlight M. chamissois chloroform partition as a promising component and may guide the search for the development of additional new anticancer therapies.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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