Zymosan-Induced Murine Peritonitis Is Associated with an Increased Sphingolipid Synthesis without Changing the Long to Very Long Chain Ceramide Ratio.

Autor: Pierron A; Interactions Hôtes-Agents Pathogènes (IHAP), Université de Toulouse, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National de la Recherche Agronomique et Environnement (INRAE), 31300 Toulouse, France., Guzylack-Piriou L; Interactions Hôtes-Agents Pathogènes (IHAP), Université de Toulouse, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National de la Recherche Agronomique et Environnement (INRAE), 31300 Toulouse, France., Tardieu D; Interactions Hôtes-Agents Pathogènes (IHAP), Université de Toulouse, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National de la Recherche Agronomique et Environnement (INRAE), 31300 Toulouse, France., Foucras G; Interactions Hôtes-Agents Pathogènes (IHAP), Université de Toulouse, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National de la Recherche Agronomique et Environnement (INRAE), 31300 Toulouse, France., Guerre P; Interactions Hôtes-Agents Pathogènes (IHAP), Université de Toulouse, Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National de la Recherche Agronomique et Environnement (INRAE), 31300 Toulouse, France.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2023 Feb 01; Vol. 24 (3). Date of Electronic Publication: 2023 Feb 01.
DOI: 10.3390/ijms24032773
Abstrakt: Sphingolipids are key molecules in inflammation and defense against pathogens. Their role in dectin-1/TLR2-mediated responses is, however, poorly understood. This study investigated the sphingolipidome in the peritoneal fluid, peritoneal cells, plasma, and spleens of mice after intraperitoneal injection of 0.1 mg zymosan/mouse or PBS as a control. Samples were collected at 2, 4, 8, and 16 h post-injection, using a total of 36 mice. Flow cytometry analysis of peritoneal cells and measurement of IL-6, IL-1β, and TNF-α levels in the peritoneal lavages confirmed zymosan-induced peritonitis. The concentrations of sphingoid bases, dihydroceramides, ceramides, dihydrosphingomyelins, sphingomyelins, monohexosylceramides, and lactosylceramides were increased after zymosan administration, and the effects varied with the time and the matrix measured. The greatest changes occurred in peritoneal cells, followed by peritoneal fluid, at 8 h and 4 h post-injection, respectively. Analysis of the sphingolipidome suggests that zymosan increased the de novo synthesis of sphingolipids without change in the C14-C18:C20-C26 ceramide ratio. At 16 h post-injection, glycosylceramides remained higher in treated than in control mice. A minor effect of zymosan was observed in plasma, whereas sphinganine, dihydrosphingomyelins, and monohexosylceramides were significantly increased in the spleen 16 h post-injection. The consequences of the observed changes in the sphingolipidome remain to be established.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje