| Autor: |
Gerzsenyi TB; Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary., Ilosvai ÁM; Institute of Chemistry, Faculty of Materials and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary., Szilágyi G; Institute of Chemistry, Faculty of Materials and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary., Szőri M; Institute of Chemistry, Faculty of Materials and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary., Váradi C; Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary., Viskolcz B; Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary.; Institute of Chemistry, Faculty of Materials and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary., Vanyorek L; Institute of Chemistry, Faculty of Materials and Chemical Engineering, University of Miskolc, 3515 Miskolc, Hungary., Szőri-Dorogházi E; Higher Education and Industrial Cooperation Centre, University of Miskolc, 3515 Miskolc, Hungary. |
| Abstrakt: |
A simplified, fast, and effective production method has been developed for the synthesis of manganese ferrite (MnFe 2 O 4 ) magnetic nanoparticles (MNPs). In addition to the wide applicability of MnFe 2 O 4 MNPs, this work also reports their application in DNA isolation for the first time. An ultrasonic-cavitation-assisted combustion method was applied in the synthesis of MnFe 2 O 4 MNPs at different furnace temperatures (573 K, 623 K, 673 K, and 773 K) to optimize the particles' properties. It was shown that MnFe 2 O 4 nanoparticles synthesized at 573 K consist of a spinel phase only with adequate size and zeta potential distributions and superparamagnetic properties. It was also demonstrated that superparamagnetic manganese ferrite nanoparticles bind DNA in buffer with a high NaCl concentration (2.5 M), and the DNA desorbs from the MNPs by decreasing the NaCl concentration of the elution buffer. This resulted in a DNA yield comparable to that of commercial DNA extraction products. Both the DNA concentration measurements and electrophoresis confirmed that a high amount of isolated bacterial plasmid DNA (pDNA) with adequate purity can be extracted with MnFe 2 O 4 (573 K) nanoparticles by applying the DNA extraction method proposed in this article. |
