| Autor: |
Moreira SSJ; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil., Silva AM; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil., Pereira AG; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil., Santos RP; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil., Dantas MRT; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil., Souza-Júnior JBF; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil., Snoeck PPN; Laboratory of Animal Reproduction, State University of Santa Cruz-UESC, Ilhéus 45662-900, Brazil., Silva AR; Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil. |
| Abstrakt: |
We evaluated the effects of detergents based on sodium dodecyl sulfoxide (SDS) on the functional parameters of collared peccary frozen-thawed sperm. Semen aliquots from ten individuals were diluted in a Tris-egg yolk-glycerol extender alone or with 0.5% Equex STM ® paste or SDS (at 0.1%, 0.3% or 0.5% ( v/v ) concentration). Samples were fast frozen in liquid nitrogen with a post-thaw evaluation of motility, membrane functionality and integrity, mitochondrial activity, sperm binding ability and thermal resistance. The treatments without SDS (41.8 ± 3.5%) and those containing Equex (41.8 ± 4.4%) or 0.1% SDS (41.2 ± 5.5%) provided greater sperm motility ( p < 0.05) than those containing SDS 0.3% (30.5 ± 4.7%) and 0.5% (31.2 ± 6.3%). Immediately after thawing, only treatments containing 0.1% SDS effectively preserved sperm straightness (STR) when compared to the negative control. All treatments preserved the amplitude of lateral head (ALH) and straightness (STR) during a thermal resistance test ( p > 0.05), but SDS 0.5% impaired the membrane functionality and mitochondrial activity after thawing ( p < 0.05). All treatments provided a similar recovery of sperm binding ability after thawing ( p < 0.05). Our results showed that the addition of 0.1% SDS to the Tris-yolk-glycerol extender optimized the freeze-thaw recovery of peccary semen. |
