| Autor: |
Abo-Almagd EE; Oncology Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., Sabala RF; Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Abd-Elghany SM; Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Jackson CR; Bacterial Epidemiology and Antimicrobial Resistance Research Unit, US National Poultry Research Center, USDA-ARS, Athens, GA 30605, USA., Ramadan H; Hygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt., Imre K; Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences 'King Mihai I' from Timișoara, 300645 Timișoara, Romania., Morar A; Department of Animal Production and Veterinary Public Health, Faculty of Veterinary Medicine, University of Life Sciences 'King Mihai I' from Timișoara, 300645 Timișoara, Romania., Herman V; Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences 'King Mihai I' from Timişoara, 300645 Timișoara, Romania., Sallam KI; Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt. |
| Abstrakt: |
Escherichia coli with multidrug resistance and β-lactamase genes may constitute a great public health hazard due to the potential for their transmission to humans through the food chain. This study determined the prevalence, antibiotic resistance profiles, phylogroups, and β-lactamase genes of E. coli isolates from chicken carcasses marketed in Mansoura, Egypt. Interestingly, E. coli was detected in 98% (98/100) of the chicken carcasses examined, which seemed among the highest contamination rates by E. coli worldwide. From the 425 genetically verified uidA gene-positive E . coli , 85 isolates were further studied for antimicrobial resistance profiles, phylogroups, and β-lactamase genes. Interestingly, 89.41% of E. coli (76/85) strains tested against 24 different antibiotics were multidrug-resistant. Of the examined 85 E. coli isolates, 22 (25.88%) isolates harbored bla CTX-M and were resistant to ampicillin, cefazoline, and ceftriaxone, while three of them were resistant to ceftazidime besides. Nine (10.59%) E. coli strains harbored AmpC- β-lactamase bla CMY and were resistant to ampicillin. One isolate co-carried bla CMY and bla CTX-M genes, though it was negative for the bla TEM gene. Of the 35 isolates that harbored either extended-spectrum β-lactamase (ESBL) and/or AmpC β-lactamase genes, six strains (17.14%) were assigned to pathogenic phylogroup F and one to phylogroup E, whereas 28 (80%) isolates belonged to commensal phylogenetic groups. |
