Comparative proteomics of saliva of healthy and gingivitis individuals from Rio de Janeiro.

Autor: da Silva CVF; Faculdade de Ciências Biológicas e da Saúde, Universidade do Estado do Rio de Janeiro, Campus Zona Oeste (UERJ-ZO), Rio de Janeiro, RJ, Brazil.; Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., Bacila Sade Y; Instituto Nacional de Metrologia Qualidade e Tecnologia (INMETRO), Duque de Caxias, RJ, Brazil., Naressi Scapin SM; Instituto Nacional de Metrologia Qualidade e Tecnologia (INMETRO), Duque de Caxias, RJ, Brazil., da Silva-Boghossian CM; Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil., de Oliveira Santos E; Faculdade de Ciências Biológicas e da Saúde, Universidade do Estado do Rio de Janeiro, Campus Zona Oeste (UERJ-ZO), Rio de Janeiro, RJ, Brazil.; Programa da Pós-graduação em Biomedicina Translacional, Unigranrio-INMETRO-UERJ-ZO, Duque de Caxias, Brazil.
Jazyk: angličtina
Zdroj: Proteomics. Clinical applications [Proteomics Clin Appl] 2023 Sep; Vol. 17 (5), pp. e2200098. Date of Electronic Publication: 2023 Feb 17.
DOI: 10.1002/prca.202200098
Abstrakt: Purpose: In this work, we identified human and bacterial proteomes in the saliva from volunteers with gingivitis or healthy.
Experimental Design: The reported population consisted of 18 volunteers (six with gingivitis and 12 healthy controls). Proteomics characterization was performed using a quantitative mass spectrometry method.
Results: A total of 74 human and 116 bacterial proteins were identified in saliva. The major functional category that was modified in the human proteome was the immune response, followed by transport and protease inhibition. In the bacterial proteome, most of the proteins identified were from the Fusobacteria phylum, followed by Chlamydiae and Spirochaetes.
Conclusions and Clinical Relevance: We observed statistically relevant differences in the data between the groups. The 15 most important human proteins affecting the variation between case and control groups included cystatin S, alpha amylase, lactotransferrin, and negative elongation factor E. We found that bacterial proteins from Porphyromonas gingivalis and Fusobacterium nucleatum subsp. nucleatum related to the red and orange complexes were closely correlated with the occurrence of periodontal diseases.
(© 2023 Wiley-VCH GmbH.)
Databáze: MEDLINE
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