Hepatitis A virus detection by RT-qPCR in shellfish samples from three Moroccan Atlantic coastal areas: Dakhla, Oualidia, and Moulay Bousselham.

Autor:	Boussettine R; Laboratory of Virology, Oncology and Biotechnology/Laboratory of Virology, Oncology, Biosciences, Environment and New Energies - Faculty of Sciences & Technologies Mohammedia - University Hassan II of Casablanca, BP 146 Mohammedia, 20650, Morocco., Hassou N; Laboratory of Virology, Oncology and Biotechnology/Laboratory of Virology, Oncology, Biosciences, Environment and New Energies - Faculty of Sciences & Technologies Mohammedia - University Hassan II of Casablanca, BP 146 Mohammedia, 20650, Morocco.; Health and Environment Laboratory, Faculty of Sciences Ain Chock, University Hassan II, B.P. 5366 Maârif, 20100 Casablanca, Morocco., Maanan M; Geosciences Laboratory, Earth Sciences Department, Faculty of Sciences Ain Chock, University Hassan II, B.P. 5366 Maârif, 20100 Casablanca, Morocco.; Polydisciplinary Faculty of Khouribga, Sultan Moulay Slimane University of Beni Mellal, BP 145 Khouribga Principal 25000, Morocco., Bessi H; Laboratory of Virology, Oncology and Biotechnology/Laboratory of Virology, Oncology, Biosciences, Environment and New Energies - Faculty of Sciences & Technologies Mohammedia - University Hassan II of Casablanca, BP 146 Mohammedia, 20650, Morocco., Ennaji MM; Laboratory of Virology, Oncology and Biotechnology/Laboratory of Virology, Oncology, Biosciences, Environment and New Energies - Faculty of Sciences & Technologies Mohammedia - University Hassan II of Casablanca, BP 146 Mohammedia, 20650, Morocco.
Jazyk:	angličtina
Zdroj:	Letters in applied microbiology [Lett Appl Microbiol] 2023 Feb 16; Vol. 76 (2).
DOI:	10.1093/lambio/ovac059
Abstrakt:	This comparative study assessed hepatitis A virus (HAV) contamination in shellfish harvesting areas in Morocco, and the correlations between viral contamination and rainfall. To this aim, HAV contamination was evaluated in 156 shellfish samples collected at three Moroccan coastal areas (52 samples/area): Dakhla (class A), Oualidia (class B), and Moulay Bousselham (class C). Samples were collected monthly between March 2018 and March 2019, and included oysters from different farms at the Oualidia and Dakhla coastal areas, and wild mussels at the Moulay Bousselham lagoon. HAV was detected by RT-qPCR in 24/156 (15.38%) samples: 16/145 (11.03%) mussel (Mytillus galloprovincialis) and 8/156 (5.13%) oyster (Crassostea gigas) samples. The 16 mussel-positive samples (16/52) were collected at Moulay Bousselham, and the eight oyster-positive samples (8/52) were collected at Oualidia, and none at Dakhla (class A). The highest HAV contamination rate was recorded at Moulay Bousselham lagoon (class C) (30.77%), while it did not exceed 8% at Oualidia (class A). A nonsignificant, positive correlation between HAV-positive samples and mean rainfall was observed. The important HAV presence in bivalve mollusks from the Oualidia and Moulay Bousselham lagoons indicate the need of viral surveillance of bivalve mollusks and of improving wastewater quality to enhance shellfish safety. (© The Author(s) 2022. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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