A compendium of single extracellular vesicle flow cytometry.

Autor: Welsh JA; Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA., Arkesteijn GJA; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Bremer M; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Cimorelli M; Vesicle Observation Center, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Experimental Clinical Chemistry, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Department of Chemical Engineering, Drexel University, Philadelphia, Pennsylvania, USA., Dignat-George F; Aix Marseille Univ, INSERM, INRAE, C2VN, UFR de Pharmacie, Marseille, France.; Hematology and Vascular Biology Department, CHU La Conception, Assistance Publique-Hôpitaux de Marseille, Marseille, France., Giebel B; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Görgens A; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.; Clinical Research Center, Department for Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.; Evox Therapeutics Ltd, Oxford, UK., Hendrix A; Laboratory of Experimental Cancer Research, Department of Human Structure and Repair, Ghent University, Ghent, Belgium., Kuiper M; Vesicle Observation Center, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Experimental Clinical Chemistry, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Biomedical Engineering & Physics, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Dutch Metrology Institute, VSL, Delft, The Netherlands., Lacroix R; Aix Marseille Univ, INSERM, INRAE, C2VN, UFR de Pharmacie, Marseille, France.; Hematology and Vascular Biology Department, CHU La Conception, Assistance Publique-Hôpitaux de Marseille, Marseille, France., Lannigan J; Flow Cytometry Support Services, LLC, Arlington, Virginia, USA., van Leeuwen TG; Vesicle Observation Center, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Biomedical Engineering & Physics, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Amsterdam Cardiovascular Sciences, Atherosclerosis and Ischemic Syndromes, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Imaging and Biomarkers, Amsterdam, The Netherlands., Lozano-Andrés E; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Rao S; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Robert S; Aix Marseille Univ, INSERM, INRAE, C2VN, UFR de Pharmacie, Marseille, France.; Hematology and Vascular Biology Department, CHU La Conception, Assistance Publique-Hôpitaux de Marseille, Marseille, France., de Rond L; Vesicle Observation Center, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Experimental Clinical Chemistry, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Biomedical Engineering & Physics, Amsterdam University Medical Centers, Amsterdam, The Netherlands., Tang VA; Flow Cytometry & Virometry Core Facility, Faculty of Medicine, Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada., Tertel T; Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany., Yan X; MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, People's Republic of China., Wauben MHM; Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands., Nolan JP; Scintillon Institute, San Diego, California, USA.; Cellarcus Biosciences, San Diego, California, USA., Jones JC; Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA., Nieuwland R; Vesicle Observation Center, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Experimental Clinical Chemistry, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Amsterdam Cardiovascular Sciences, Atherosclerosis and Ischemic Syndromes, Amsterdam, The Netherlands., van der Pol E; Vesicle Observation Center, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Experimental Clinical Chemistry, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Biomedical Engineering & Physics, Amsterdam University Medical Centers, Amsterdam, The Netherlands.; Amsterdam Cardiovascular Sciences, Atherosclerosis and Ischemic Syndromes, Amsterdam, The Netherlands.; Cancer Center Amsterdam, Imaging and Biomarkers, Amsterdam, The Netherlands.
Jazyk: angličtina
Zdroj: Journal of extracellular vesicles [J Extracell Vesicles] 2023 Feb; Vol. 12 (2), pp. e12299.
DOI: 10.1002/jev2.12299
Abstrakt: Flow cytometry (FCM) offers a multiparametric technology capable of characterizing single extracellular vesicles (EVs). However, most flow cytometers are designed to detect cells, which are larger than EVs. Whereas cells exceed the background noise, signals originating from EVs partly overlap with the background noise, thereby making EVs more difficult to detect than cells. This technical mismatch together with complexity of EV-containing fluids causes limitations and challenges with conducting, interpreting and reproducing EV FCM experiments. To address and overcome these challenges, researchers from the International Society for Extracellular Vesicles (ISEV), International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH) joined forces and initiated the EV FCM working group. To improve the interpretation, reporting, and reproducibility of future EV FCM data, the EV FCM working group published an ISEV position manuscript outlining a framework of minimum information that should be reported about an FCM experiment on single EVs (MIFlowCyt-EV). However, the framework contains limited background information. Therefore, the goal of this compendium is to provide the background information necessary to design and conduct reproducible EV FCM experiments. This compendium contains background information on EVs, the interaction between light and EVs, FCM hardware, experimental design and preanalytical procedures, sample preparation, assay controls, instrument data acquisition and calibration, EV characterization, and data reporting. Although this compendium focuses on EVs, many concepts and explanations could also be applied to FCM detection of other particles within the EV size range, such as bacteria, lipoprotein particles, milk fat globules, and viruses.
(© 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.)
Databáze: MEDLINE
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