Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence.

Autor: Semenec L; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia.; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Cain AK; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia.; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Dawson CJ; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia., Liu Q; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Dinh H; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia.; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Lott H; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Penesyan A; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia.; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Maharjan R; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia.; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia., Short FL; Department of Microbiology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, 3800, Australia., Hassan KA; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia. karl.hassan@newcastle.edu.au.; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia. karl.hassan@newcastle.edu.au., Paulsen IT; ARC Centre of Excellence in Synthetic Biology, School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia. ian.paulsen@mq.edu.au.; School of Natural Sciences, Macquarie University, North Ryde, NSW, 2113, Australia. ian.paulsen@mq.edu.au.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Feb 09; Vol. 14 (1), pp. 702. Date of Electronic Publication: 2023 Feb 09.
DOI: 10.1038/s41467-023-36252-2
Abstrakt: Acinetobacter baumannii and Klebsiella pneumoniae are opportunistic pathogens frequently co-isolated from polymicrobial infections. The infections where these pathogens co-exist can be more severe and recalcitrant to therapy than infections caused by either species alone, however there is a lack of knowledge on their potential synergistic interactions. In this study we characterise the genomes of A. baumannii and K. pneumoniae strains co-isolated from a single human lung infection. We examine various aspects of their interactions through transcriptomic, phenomic and phenotypic assays that form a basis for understanding their effects on antimicrobial resistance and virulence during co-infection. Using co-culturing and analyses of secreted metabolites, we discover the ability of K. pneumoniae to cross-feed A. baumannii by-products of sugar fermentation. Minimum inhibitory concentration testing of mono- and co-cultures reveals the ability for A. baumannii to cross-protect K. pneumoniae against the cephalosporin, cefotaxime. Our study demonstrates distinct syntrophic interactions occur between A. baumannii and K. pneumoniae, helping to elucidate the basis for their co-existence in polymicrobial infections.
(© 2023. The Author(s).)
Databáze: MEDLINE
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