C5aR1 activation in mice controls inflammatory eosinophil recruitment and functions in allergic asthma.

Autor: Wiese AV; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany., Duhn J; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany., Korkmaz RÜ; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany., Quell KM; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany., Osman I; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany., Ender F; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany., Schröder T; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany.; Institute of Nutritional Medicine, University Hospital of Schleswig-Holstein & University of Lübeck, Lübeck, Germany., Lewkowich I; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA., Hogan S; Mary H. Weiser Food Allergy Center, Experimental Pathology, Department of Pathology, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA., Huber-Lang M; Institute of Clinical and Experimental Trauma-Immunology (ITI), University of Ulm, Ulm, Germany., Gumprecht F; Institute for Anatomy, University of Lübeck, Lübeck, Germany., König P; Institute for Anatomy, University of Lübeck, Lübeck, Germany.; Airway Research Center North, Member of the German Center for Lung Research (DZL), Lübeck, Germany., Köhl J; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany.; Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.; Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA.; Airway Research Center North, Member of the German Center for Lung Research (DZL), Lübeck, Germany., Laumonnier Y; Institute for Systemic Inflammation Research, University of Lübeck, Lübeck, Germany.; Institute of Nutritional Medicine, University Hospital of Schleswig-Holstein & University of Lübeck, Lübeck, Germany.; Airway Research Center North, Member of the German Center for Lung Research (DZL), Lübeck, Germany.
Jazyk: angličtina
Zdroj: Allergy [Allergy] 2023 Jul; Vol. 78 (7), pp. 1893-1908. Date of Electronic Publication: 2023 Feb 21.
DOI: 10.1111/all.15670
Abstrakt: Background: Pulmonary eosinophils comprise at least two distinct populations of resident eosinophils (rEOS) and inflammatory eosinophils (iEOS), the latter recruited in response to pulmonary inflammation. Here, we determined the impact of complement activation on rEOS and iEOS trafficking and function in two models of pulmonary inflammation.
Methods: BALB/c wild-type and C5ar1 -/- mice were exposed to different allergens or IL-33. Eosinophil populations in the airways, lung, or mediastinal lymph nodes (mLN) were characterized by FACS or immunohistochemistry. rEOS and iEOS functions were determined in vivo and in vitro.
Results: HDM and IL-33 exposure induced a strong accumulation of iEOS but not rEOS in the airways, lungs, and mLNs. rEOS and iEOS expressed C3/C5 and C5aR1, which were significantly higher in iEOS. Initial pulmonary trafficking of iEOS was markedly reduced in C5ar1 -/- mice and associated with less IL-5 production from ILC2 cells. Functionally, adoptively transferred pulmonary iEOS from WT but not from C5ar1 -/- mice-induced airway hyperresponsiveness (AHR), which was associated with significantly reduced C5ar1 -/- iEOS degranulation. Pulmonary iEOS but not rEOS were frequently associated with T cells in lung tissue. After HDM or IL-33 exposure, iEOS but not rEOS were found in mLNs, which were significantly reduced in C5ar1 -/- mice. C5ar1 -/- iEOS expressed less costimulatory molecules, associated with a decreased potency to drive antigen-specific T cell proliferation and differentiation into memory T cells.
Conclusions: We uncovered novel roles for C5aR1 in iEOS trafficking and activation, which affects key aspects of allergic inflammation such as AHR, ILC2, and T cell activation.
(© 2023 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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