An electrochemical aptasensor based on AuNRs/AuNWs for sensitive detection of apolipoprotein A-1 (ApoA1) from human serum.

Autor: Husna R; Biosensors and Nanobiotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam minhaz.ahmed@ubd.edu.bn minhaz.ahmed@outlook.com., Kurup CP; PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam., Ansari MA; Biosensors and Nanobiotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam minhaz.ahmed@ubd.edu.bn minhaz.ahmed@outlook.com., Mohd-Naim NF; Biosensors and Nanobiotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam minhaz.ahmed@ubd.edu.bn minhaz.ahmed@outlook.com.; PAPRSB Institute of Health Sciences, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam., Ahmed MU; Biosensors and Nanobiotechnology Laboratory, Integrated Science Building, Faculty of Science, Universiti Brunei Darussalam Jalan Tungku Link Gadong BE 1410 Brunei Darussalam minhaz.ahmed@ubd.edu.bn minhaz.ahmed@outlook.com.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2023 Jan 26; Vol. 13 (6), pp. 3890-3898. Date of Electronic Publication: 2023 Jan 26 (Print Publication: 2023).
DOI: 10.1039/d2ra06600a
Abstrakt: For early detection and diagnosis of cancer, it is essential to develop an electrochemical biosensor that is quick, accurate, and sensitive. Here, we use gold nanorod (AuNR) and gold nanowire (AuNW) nanocomposites (AuNR/AuNW/CS) as electrode modifiers on a glassy carbon electrode (GCE) to construct a sensitive label-free electrochemical aptasensor to detect ApoA1. The thiolated ApoA1-specific aptamers were immobilized onto the modified electrode surface through self-assembled monolayers. Electrochemical techniques, such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV), were used to analyze the fabrication steps. The concentration of ApoA1 was measured with DPV on the aptasensor, with a linear range of 0.1 to 1000 pg mL -1 and a detection limit of 0.04 pg mL -1 . When compared to results from ELISA tests (which have a detection limit of 80 pg mL -1 ), the results achieved here were over 2000 times better. The aptasensor's performance was successfully evaluated using human serum spiked with ApoA1, suggesting that it has great potential for practical application. The electrochemical apatsensor additionally demonstrated outstanding selectivity responses and strong stability toward the target analyte.
Competing Interests: The authors have no conflicts of interest to declare. All co-authors have seen and agree with the contents of the manuscript and there is no financial interest to report. We certify that the submission is original work and is not under review at any other publication.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE