Heat-induced proteomic changes in anthers of contrasting rice genotypes under variable stress regimes.

Autor:	Kumar R; Department of Plant Molecular Biology, University of Delhi, New Delhi, India., Ghatak A; Molecular Systems Biology Lab (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Vienna, Austria., Goyal I; Department of Plant Molecular Biology, University of Delhi, New Delhi, India., Sarkar NK; Department of Plant Molecular Biology, University of Delhi, New Delhi, India., Weckwerth W; Molecular Systems Biology Lab (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Vienna, Austria.; Vienna Metabolomics Center (VIME), University of Vienna, Vienna, Austria., Grover A; Department of Plant Molecular Biology, University of Delhi, New Delhi, India., Chaturvedi P; Molecular Systems Biology Lab (MOSYS), Department of Functional and Evolutionary Ecology, University of Vienna, Vienna, Austria.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2023 Jan 13; Vol. 13, pp. 1083971. Date of Electronic Publication: 2023 Jan 13 (Print Publication: 2022).
DOI:	10.3389/fpls.2022.1083971
Abstrakt:	Heat stress drastically affects anther tissues resulting in poor plant fertility, necessitating an urgent need to determine the key proteome regulation associated with mature anther in response to heat stress. We identified several genotype - specific protein alterations in rice anthers of Moroberekan (Japonica, heat sensitive), IR64 (Indica, moderately heat tolerant), and Nagina22 (Aus, heat tolerant) in the short-term (ST_HS; one cycle of 42°C, 4 hours before anthesis) and long-term (LT_HS; 6 cycles of 38°C, 6 hours before anthesis) heat stress. The proteins upregulated in long-term heat stress in Nagina22 were enriched in biological processes related to unfolded protein binding and carboxylic acid metabolism, including amino acid metabolism. In short-term heat stress, Nagina22 anthers were enriched in proteins associated with vitamin E biosynthesis and GTPase activator activity. In contrast, downregulated proteins were related to ribosomal proteins. The expression of different Hsp20 and DnaJ was genotype specific. Overall, the heat response in Nagina22 was associated with its capacity for adequate metabolic control and cellular homeostasis, which may be critical for its higher reproductive thermotolerance. This study improves our understanding of thermotolerance mechanisms in rice anthers during anthesis and lays a foundation for breeding thermotolerant varieties via molecular breeding. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Kumar, Ghatak, Goyal, Sarkar, Weckwerth, Grover and Chaturvedi.)
Databáze:	MEDLINE
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