Pleural fluid microbiota as a biomarker for malignancy and prognosis.

Autor: Kwok B; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA., Wu BG; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA.; Division of Pulmonary and Critical Care Medicine, Veterans Affairs New York Harbor Healthcare System, New York, NY, USA., Kocak IF; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA., Sulaiman I; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA.; Department of Respiratory Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland.; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland., Schluger R; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA., Li Y; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA., Anwer R; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA., Goparaju C; Department of Cardiothoracic Surgery, New York University Grossman School of Medicine, New York, NY, USA., Ryan DJ; Department of Respiratory Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland.; Department of Respiratory Medicine, Beaumont Hospital, Dublin, Ireland., Sagatelian M; School of Medicine, Medical University of South Carolina, Charleston, SC, USA., Dreier MS; New York University Grossman School of Medicine, New York, NY, USA., Murthy V; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA.; Department of Cardiothoracic Surgery, New York University Grossman School of Medicine, New York, NY, USA., Rafeq S; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA.; Department of Cardiothoracic Surgery, New York University Grossman School of Medicine, New York, NY, USA., Michaud GC; Division of Pulmonary, Critical Care, and Sleep Medicine, University of South Florida Health, Tampa, FL, USA., Sterman DH; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA.; Department of Cardiothoracic Surgery, New York University Grossman School of Medicine, New York, NY, USA., Bessich JL; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA.; Department of Cardiothoracic Surgery, New York University Grossman School of Medicine, New York, NY, USA., Pass HI; Department of Cardiothoracic Surgery, New York University Grossman School of Medicine, New York, NY, USA., Segal LN; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA., Tsay JJ; Division of Pulmonary, Critical Care, and Sleep Medicine, New York University Grossman School of Medicine, 462 First Avenue 7N21, New York, NY, 10016, USA. Jun-chieh.Tsay@nyulangone.org.; Division of Pulmonary and Critical Care Medicine, Veterans Affairs New York Harbor Healthcare System, New York, NY, USA. Jun-chieh.Tsay@nyulangone.org.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Feb 08; Vol. 13 (1), pp. 2229. Date of Electronic Publication: 2023 Feb 08.
DOI: 10.1038/s41598-023-29001-4
Abstrakt: Malignant pleural effusions (MPE) complicate malignancies and portend worse outcomes. MPE is comprised of various components, including immune cells, cancer cells, and cell-free DNA/RNA. There have been investigations into using these components to diagnose and prognosticate MPE. We hypothesize that the microbiome of MPE is unique and may be associated with diagnosis and prognosis. We compared the microbiota of MPE against microbiota of pleural effusions from non-malignant and paramalignant states. We collected a total of 165 pleural fluid samples from 165 subjects; Benign (n = 16), Paramalignant (n = 21), MPE-Lung (n = 57), MPE-Other (n = 22), and Mesothelioma (n = 49). We performed high throughput 16S rRNA gene sequencing on pleural fluid samples and controls. We showed that there are compositional differences among pleural effusions related to non-malignant, paramalignant, and malignant disease. Furthermore, we showed differential enrichment of bacterial taxa within MPE depending on the site of primary malignancy. Pleural fluid of MPE-Lung and Mesothelioma were associated with enrichment with oral and gut bacteria that are commonly thought to be commensals, including Rickettsiella, Ruminococcus, Enterococcus, and Lactobacillales. Mortality in MPE-Lung is associated with enrichment in Methylobacterium, Blattabacterium, and Deinococcus. These observations lay the groundwork for future studies that explore host-microbiome interactions and their influence on carcinogenesis.
(© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)
Databáze: MEDLINE
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