Antifungal effect of the liposome encapsulation of the Trans- Caryophylene and its association with fluconazole.

Autor: Bezerra CF; Department of Pharmaceutical Sciences, Federal University of Pernambuco- UFPE, Recife, PE, Brazil., Geraldo de Alencar Júnior J; Department of Pharmacy, Federal University of Ceará- UFC, Fortaleza, CE, Brazil., de Lima Honorato R; Department of Biological Sciences, Regional University of Cariri- URCA, Crato, CE, Brazil., Lucas Dos Santos AT; Department of Biological Sciences, Regional University of Cariri- URCA, Crato, CE, Brazil., Pereira da Silva JC; Department of Biological Sciences, Regional University of Cariri- URCA, Crato, CE, Brazil., Gusmão da Silva T; Department of Biological Sciences, Regional University of Cariri- URCA, Crato, CE, Brazil., Sampaio de Freitas T; Department of Biological Chemistry, Regional University of Cariri- URCA, Crato, CE, Brazil., Fonseca Bezerra MC; Faculty of Medicine of Juazeiro do Norte- Estácio FMJ, Juazeiro do Norte, CE, Brazil., Alves Borges Leal AL; Department of Biological Chemistry, Regional University of Cariri- URCA, Crato, CE, Brazil., Sales DL; Department of Biological Chemistry, Regional University of Cariri- URCA, Crato, CE, Brazil., Viana Rodrigues JP; Department of Pharmacy, Federal University of Ceará- UFC, Fortaleza, CE, Brazil., Barbosa Filho JM; Department of Pharmacy, Federal University of Paraíba- UFPB, João Pessoa, PB, Brazil., Peixoto LR; Department of Pharmacy, Federal University of Paraíba- UFPB, João Pessoa, PB, Brazil., Pinheiro AP; Department of Biological Sciences, Regional University of Cariri- URCA, Crato, CE, Brazil., Melo Coutinho HD; Department of Biological Chemistry, Regional University of Cariri- URCA, Crato, CE, Brazil. Electronic address: hdmcoutinho@urca.br., Bezerra Morais-Braga MF; Department of Biological Sciences, Regional University of Cariri- URCA, Crato, CE, Brazil., Gonçalves da Silva T; Department of Antibiotics, Federal University of Pernambuco- UFPE, Recife, PE, Brazil.
Jazyk: angličtina
Zdroj: Chemico-biological interactions [Chem Biol Interact] 2023 Mar 01; Vol. 373, pp. 110377. Date of Electronic Publication: 2023 Feb 06.
DOI: 10.1016/j.cbi.2023.110377
Abstrakt: Trans-Caryophyllene (TC), a sesquiterpene, with proven biological activities, which in this work was tested alone, encapsulated in liposomes and associated with Fluconazole in vitro in an attempt to enhance the effect of the drug. Liposomes were characterized from vesicle size, polydispersity index, and Zeta potential, and imaging by scanning electron microscopy. Antifungal assays were performed against Candida albicans, Candida tropicalis and Candida krusei by microdilution to determine the IC 50 values and the viability curve. The Minimum Fungicidal Concentration (MFC) was performed by subcultivation in solid medium and the inhibitory effect of the association of TC and Fluconazole and tests to verify morphological changes was performed in micro-cultivation chambers based on concentrations on microdilution plates. The corresponding IC 50 data of the substances ranged from 34.4 to 65249 μg/mL, considerably high values compared to the control (Fluconazole). The MFC of all compounds showing fungistatic effect. The performance of the compounds on the cell viability curve was similar in all tested strains, as they showed no antifungal potential when compared to the control (FCZ), when associated with FCZ they showed no significant antifungal activity. The free and liposomal TC also managed to restrict 100% of the fungal dimorphism, in both concentrations, against C. albicans, and against C. tropicalis the isolated TC did not show a significant inhibitory effect; however, against the C. krusei strain inhibited 100% in filamentous growth in both concentrations, which is statistically relevant. The liposomes were homogeneous, with vesicles with diameters of 185.46 nm for the control and 143.8 nm for the liposomal TC, and a surface charge potential of - 42.6 mV. By scanning microscopy, the spherical shapes of the vesicles were verified.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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