Formation of Listeria monocytogenes persister cells in the produce-processing environment.

Autor: Li X; Department of Food Science and Technology, University of California Davis, Davis, CA 95616, United States., Hospital XF; Department of Food Technology, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain., Hierro E; Department of Food Technology, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain., Fernández M; Department of Food Technology, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain., Sheng L; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214126, China., Wang L; Department of Food Science and Technology, University of California Davis, Davis, CA 95616, United States. Electronic address: lxwang@ucdavis.edu.
Jazyk: angličtina
Zdroj: International journal of food microbiology [Int J Food Microbiol] 2023 Apr 02; Vol. 390, pp. 110106. Date of Electronic Publication: 2023 Jan 26.
DOI: 10.1016/j.ijfoodmicro.2023.110106
Abstrakt: Persisters are a subpopulation of growth-arrested cells that possess transient tolerance to lethal doses of antibiotics and can revert to an active state under the right conditions. Persister cells are considered as a public health concern. This study examined the formation of persisters by Listeria monocytogenes (LM) in an environment simulating a processing plant for leafy green production. Three LM strains isolated from California produce-processing plants and packinghouses with the strongest adherence abilities were used for this study. The impact of the cells' physiological status, density, and nutrient availability on the formation of persisters was also determined. Gentamicin at a dose of 100 mg/L was used for the isolation and screening of LM persisters. Results showed that the physiological status differences brought by culture preparation methods (plate-grown vs. broth-grown) did not impact persister formation (P > 0.05). Instead, higher persister ratios were found when cell density increased (P < 0.05). The formation of LM persister cells under simulated packinghouse conditions was tested by artificially inoculating stainless steel coupons with LM suspending in media with decreasing nutrient levels: brain heart infusion broth (1366 mg/L O 2 ), produce-washing water with various organic loads (1332 mg/L O 2 and 652 mg/L O 2 , respectively), as well as sterile Milli-Q water. LM survived in all suspensions at 4 °C with 85 % relative humidity for 7 days, with strain 483 producing the most persister cells (4.36 ± 0.294 Log CFU/coupon) on average. Although persister cell levels of LM 480 and 485 were reasonably steady in nutrient-rich media (i.e., BHI and HCOD), they declined in nutrient-poor media (i.e., LCOD and sterile Milli-Q water) over time. Persister populations decreased along with total viable cells, demonstrating the impact of available nutrients on the formation of persisters. The chlorine sensitivity of LM persister cells was evaluated and compared with regular LM cells. Results showed that, despite their increased tolerance to the antibiotic gentamicin, LM persisters were more susceptible to chlorine treatments (100 mg/L for 2 min) than regular cells.
Competing Interests: Declaration of competing interest All Authors, Xiran Li, Xavier F. Hospital, Eva Hierro, Manuela Fernández, Lina Sheng, and Luxin Wang, certify that there is no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. There is no conflict of interest from any of the authors.
(Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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