| Autor: |
Shalmani AA; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Ahmed Z; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Sheybanifard M; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Wang A; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Weiler M; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Buhl EM; Electron Microscopy Facility, Institute of Pathology, RWTH University Hospital, 52074 Aachen, Germany., Klinkenberg G; Department of Biotechnology and Nanomedicine, SINTEF Industry, 7034, Trondheim, Norway., Schmid R; Department of Biotechnology and Nanomedicine, SINTEF Industry, 7034, Trondheim, Norway., Hennink W; Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3508 TB Utrecht, The Netherlands., Kiessling F; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Metselaar JM; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Lammers T; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Peña Q; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany., Shi Y; Institute for Experimental Molecular Imaging, RWTH Aachen University Hospital, 52074 Aachen, Germany. |
| Abstrakt: |
Polymeric micelles are among the most extensively used drug delivery systems. Key properties of micelles, such as size, size distribution, drug loading, and drug release kinetics, are crucial for proper therapeutic performance. Whether polymers from more controlled polymerization methods produce micelles with more favorable properties remains elusive. To address this question, we synthesized methoxy poly(ethylene glycol)- b -( N -(2-benzoyloxypropyl)methacrylamide) (mPEG- b -p(HPMAm-Bz)) block copolymers of three different comparable molecular weights (∼9, 13, and 20 kDa), via both conventional free radical (FR) and reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymers were subsequently employed to prepare empty and paclitaxel-loaded micelles. While FR polymers had relatively high dispersities ( Đ ∼ 1.5-1.7) compared to their RAFT counterparts ( Đ ∼ 1.1-1.3), they formed micelles with similar pharmaceutical properties (e.g., size, size distribution, critical micelle concentration, cytotoxicity, and drug loading and retention). Our findings suggest that pharmaceutical properties of mPEG- b -p(HPMAm-Bz) micelles do not depend on the synthesis route of their constituent polymers. |
