| Autor: |
Zhu P; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands.; State Key Laboratory of Mechanics and Control of Mechanical Structures, Nanjing University of Aeronautics and Astronautics, 210016 Nanjing, China., Papadimitriou VA; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands., van Dongen JE; BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, Technical Medical Centre, Max Planck Institute for Complex Fluid Dynamics, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands., Cordeiro J; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands., Neeleman Y; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands., Santoso A; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands., Chen S; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands.; Institute of Organic and Polymeric Materials, National Taipei University of Technology, 10608 Taipei, Taiwan.; Research and Development Center for Smart Textile Technology, National Taipei University of Technology, 10608 Taipei, Taiwan., Eijkel JCT; BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, Technical Medical Centre, Max Planck Institute for Complex Fluid Dynamics, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands., Peng H; State Key Laboratory of Mechanics and Control of Mechanical Structures, Nanjing University of Aeronautics and Astronautics, 210016 Nanjing, China., Segerink LI; BIOS Lab on a Chip Group, MESA+ Institute for Nanotechnology, Technical Medical Centre, Max Planck Institute for Complex Fluid Dynamics, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands., Rwei AY; Department of Chemical Engineering, Delft University of Technology, 2629 HZ, Delft, Netherlands. |
| Abstrakt: |
Endotoxin is a deadly pyrogen, rendering it crucial to monitor with high accuracy and efficiency. However, current endotoxin detection relies on multistep processes that are labor-intensive, time-consuming, and unsustainable. Here, we report an aptamer-based biosensor for the real-time optical detection of endotoxin. The endotoxin sensor exploits the distance-dependent scattering of gold nanoparticles (AuNPs) coupled to a gold nanofilm. This is enabled by the conformational changes of an endotoxin-specific aptamer upon target binding. The sensor can be used in an ensemble mode and single-particle mode under dark-field illumination. In the ensemble mode, the sensor is coupled with a microspectrometer and exhibits high specificity, reliability (i.e., linear concentration to signal profile in logarithmic scale), and reusability for repeated endotoxin measurements. Individual endotoxins can be detected by monitoring the color of single AuNPs via a color camera, achieving single-molecule resolution. This platform can potentially advance endotoxin detection to safeguard medical, food, and pharmaceutical products. |
