Release of leachable products from resinous compounds in the saliva of children with anterior open bite treated with spur.

Autor:	Tou GADA; Universidade Federal de Minas Gerais, Faculdade de Odontologia Belo Horizonte, Departamento de Odontologia Restauradora, Minas Gerais, Brasil., Gomes JM; Universidade Federal de Minas Gerais, Instituto de Ciências Exatas, Departamento de Química, Belo Horizonte, Minas Gerais, Brasil., Rinco LSO; Universidade Federal de Minas Gerais, Faculdade de Odontologia Belo Horizonte, Departamento de Odontologia Restauradora, Minas Gerais, Brasil., Yamauti M; Hokkaido University, School of Dentistry, Department of Restorative Dentistry, Sapporo, Japan., Diniz IMA; Universidade Federal de Minas Gerais, Faculdade de Odontologia Belo Horizonte, Departamento de Odontologia Restauradora, Minas Gerais, Brasil., Pires F; Universidade Estadual de Campinas, Instituto de Química, Campinas, São Paulo, Brasil., Schmidt MEP; Universidade Estadual de Campinas, Instituto de Química, Campinas, São Paulo, Brasil., Menezes HC; Universidade Federal de Minas Gerais, Instituto de Ciências Exatas, Departamento de Química, Belo Horizonte, Minas Gerais, Brasil., Cardeal ZL; Universidade Federal de Minas Gerais, Instituto de Ciências Exatas, Departamento de Química, Belo Horizonte, Minas Gerais, Brasil., Bottoli CBG; Universidade Estadual de Campinas, Instituto de Química, Campinas, São Paulo, Brasil., Macari S; Universidade Federal de Minas Gerais, Faculdade de Odontologia Belo Horizonte, Departamento de Odontologia Restauradora, Minas Gerais, Brasil.
Jazyk:	angličtina
Zdroj:	Journal of applied oral science : revista FOB [J Appl Oral Sci] 2023 Feb 03; Vol. 30, pp. e20220227. Date of Electronic Publication: 2023 Feb 03 (Print Publication: 2023).
DOI:	10.1590/1678-7757-2022-0227
Abstrakt:	Background: To evaluate the release of bisphenol-A glycidyl methacrylate (BisGMA), triethylene glycol dimethacrylate (TEGDMA), bisphenol A (BPA), and phthalates of the composite resin used in the bonding of spurs applied in the treatment of children with anterior open bite and its effects on human keratinocytes. Methodology: Saliva samples of 22 children were collected before spur attachment (baseline) and 30 minutes (min) and 24 hours (h) after spur bonding. Analysis was performed using high-performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (HPLC-MS/MS) and gas chromatography coupled to mass spectrometry (GC-MS). Standardized resin increments were added to three different dilutions of the cell culture medium. Keratinocytes (HaCaT) were cultivated in the conditioned media and evaluated for cell viability (MTT) and cell scratch assay. Results: The levels of BisGMA (1.74±0.27 μg/mL), TEGDMA (2.29±0.36 μg/mL), and BPA (3.264±0.88 μg/L) in the saliva after 30 min, in comparison to baseline (0±0 μg/mL, 0±0 μg/mL, and 1.15±0.21 μg/L, respectively), presented higher numbers. After 24 h, the levels of the monomers were similar to the baseline. Phthalates showed no significant difference among groups. HaCat cells showed increased viability and reduced cell migration over time after exposure to methacrylate-based resin composites. Conclusion: Resin composites, used to attach spurs in children with anterior open bite during orthodontic treatment, release monomers after polymerization and can influence the behavior of human keratinocytes, even at very low concentrations. Orthodontists should be aware of the risks of the resinous compounds release and preventive procedures should be held to reduce patient exposure.
Databáze:	MEDLINE
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