Construction of the advanced flavin mononucleotide producers in the flavinogenic yeast Candida famata.

Autor: Fedorovych DV; Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Lviv, Ukraine., Tsyrulnyk AO; Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Lviv, Ukraine., Ruchala J; Department of Biotechnology and Microbiology, University of Rzeszow, Rzeszow, Poland., Sobchuk SM; Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Lviv, Ukraine., Dmytruk KV; Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Lviv, Ukraine., Fayura LR; Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Lviv, Ukraine., Sibirny AA; Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Science of Ukraine, Lviv, Ukraine.; Department of Biotechnology and Microbiology, University of Rzeszow, Rzeszow, Poland.
Jazyk: angličtina
Zdroj: Yeast (Chichester, England) [Yeast] 2023 Aug; Vol. 40 (8), pp. 360-366. Date of Electronic Publication: 2023 Feb 20.
DOI: 10.1002/yea.3843
Abstrakt: Flavin mononucleotide (FMN, riboflavin-5'-phosphate) is flavin coenzyme synthesized in all living organisms from riboflavin (vitamin B 2 ) after phosphorylation in the reaction catalyzed by riboflavin kinase. FMN has several applications mostly as yellow colorant in food industry due to 200 times better water solubility as compared to riboflavin. Currently, FMN is produced by chemical phosphorylation of riboflavin, however, final product contains up to 25% of flavin impurities. Microbial overproducers of FMN are known, however, they accumulate this coenzyme in glucose medium. Current work shows that the recombinant strains of the flavinogenic yeast Candida famata with overexpressed FMN1 gene coding for riboflavin kinase in the recently isolated by us advanced riboflavin producers due to overexpression of the structural and regulatory genes of riboflavin synthesis and of the putative exporter of riboflavin from the cell, synthesized elevated amounts of FMN in the media not only with glucose but also in lactose and cheese whey. Activation of FMN accumulation on lactose and cheese whey was especially strong in the strains which expressed the gene of transcription activator SEF1 under control of the lactose-induced LAC4 promoter. The accumulation of this coenzyme by the washed cells of the best recombinant strain achieved 540 mg/L in the cheese whey supplemented only with ammonium sulfate during 48 h in shake flask experiments.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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