| Autor: |
Lopez Marin MA; University of Chemistry and Technology, Prague. Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague, Czech Republic., Suman J; University of Chemistry and Technology, Prague. Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague, Czech Republic., Jani K; University of Chemistry and Technology, Prague. Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague, Czech Republic., Ulbrich P; University of Chemistry and Technology, Prague. Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague, Czech Republic., Cajthaml T; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 14220, Prague, Czech Republic., Filipova A; Institute of Microbiology of the Czech Academy of Sciences, Videnska 1083, 14220, Prague, Czech Republic., Pajer P; Military Health Institute, Ministry of Defence of the Czech Republic, U Vojenske nemocnice 1200, 169 02, Prague, Czech Republic., Neumann-Schaal M; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH, Inhoffenstr. 7 B, 38124, Braunschweig, Germany., Strejcek M; University of Chemistry and Technology, Prague. Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague, Czech Republic., Uhlik O; University of Chemistry and Technology, Prague. Department of Biochemistry and Microbiology, Technicka 3, 16628 Prague, Czech Republic. |
| Abstrakt: |
An actinobacterial strain, designated A5X3R13 T , was isolated from a compost soil suspension supplemented with extracellular material from a Micrococcus luteus -culture supernatant. The strain was cultured on tenfold-diluted reasoner's 2A agar. The cells were ovoid-to-rod shaped, non-motile, Gram-stain-positive, oxidase-negative, catalase-positive and had a width of 0.5 µm and a length of 0.8-1.2 µm. The results of both 16S rRNA-based phylogenetic and whole-genome analyses indicate that A5X3R13 T forms a distinct lineage within the family Nocardioidaceae (order Propionibacteriales ). On the basis of the 16S rRNA gene sequence, A5X3R13 T was closely related to Aeromicrobium terrae CC-CFT486 T (96.2 %), Nocardioides iriomotensis IR27-S3 T (96.2 %), Nocardioides guangzhouensis 130 T (95.6 %), Marmoricola caldifontis YIM 730233 T (95.5 %), Aeromicrobium alkaliterrae KSL-107 T (95.4 %), Aeromicrobium choanae 9H-4 T (95.4 %), Aeromicrobium panaciterrae Gsoil 161 T (95.3 %), and Nocardioides jensenii NBRC 14755 T (95.2 %). The genome had a length of 4 915 757 bp, and its DNA G+C content was 68.5 mol %. The main fatty acids were 10-methyl C 17 : 0 , C 16 : 0 , C 15 : 0 , C 18 : 0 , C 17 : 0 and iso-C 16 : 0 . The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. MK-9(H 4 ) was the predominant respiratory quinone. The peptidoglycan type was A3γ (A41.1) and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid in a molar ratio of 1.2 : 0.9 : 1.0 : 0.8. On the basis of the results of the phylogenetic and phenotypic analyses and comparisons with other members of the family Nocardioidaceae , strain A5X3R13 T is proposed to represent a novel species within a novel genus, for which the name Solicola gregarius gen. nov., sp. nov. is proposed. The type strain is A5X3R13 T (=DSM 112953 T =NCCB 100840 T ). |
