Polarized branched Actin modulates cortical mechanics to produce unequal-size daughters during asymmetric division.

Autor: Daeden A; Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland., Mietke A; Department of Mathematics, Massachusetts Institute of Technology, Cambridge, MA, USA.; Max Planck Institute for the Physics of Complex Systems, Dresden, Germany.; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.; Center for Systems Biology Dresden, Dresden, Germany., Derivery E; Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland.; MRC Laboratory of Molecular Biology, Cambridge, UK., Seum C; Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland., Jülicher F; Max Planck Institute for the Physics of Complex Systems, Dresden, Germany., Gonzalez-Gaitan M; Department of Biochemistry, Faculty of Sciences, University of Geneva, Geneva, Switzerland. marcos.gonzalez@unige.ch.
Jazyk: angličtina
Zdroj: Nature cell biology [Nat Cell Biol] 2023 Feb; Vol. 25 (2), pp. 235-245. Date of Electronic Publication: 2023 Feb 06.
DOI: 10.1038/s41556-022-01058-9
Abstrakt: The control of cell shape during cytokinesis requires a precise regulation of mechanical properties of the cell cortex. Only few studies have addressed the mechanisms underlying the robust production of unequal-sized daughters during asymmetric cell division. Here we report that unequal daughter-cell sizes resulting from asymmetric sensory organ precursor divisions in Drosophila are controlled by the relative amount of cortical branched Actin between the two cell poles. We demonstrate this by mistargeting the machinery for branched Actin dynamics using nanobodies and optogenetics. We can thereby engineer the cell shape with temporal precision and thus the daughter-cell size at different stages of cytokinesis. Most strikingly, inverting cortical Actin asymmetry causes an inversion of daughter-cell sizes. Our findings uncover the physical mechanism by which the sensory organ precursor mother cell controls relative daughter-cell size: polarized cortical Actin modulates the cortical bending rigidity to set the cell surface curvature, stabilize the division and ultimately lead to unequal daughter-cell size.
(© 2023. The Author(s).)
Databáze: MEDLINE
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