Molecular detection of monkeypox and related viruses: challenges and opportunities.

Autor: Ghate SD; Center for Bioinformatics, NITTE Deemed to be University, Mangaluru, 575018, India. sudeep1129@gmail.com., Suravajhala P; Amrita School of Biotechnology, Amrita Vishwa Vidyapeetham, Amritapuri, Kerala, 690525, India., Patil P; Central Research Laboratory, KS Hegde Medical Academy (KSHEMA), NITTE Deemed to be University, Mangaluru, 575018, India., Vangala RK; Institute for Applied Research and Innovation, Neuome Technologies Pvt. Ltd., Bangalore Bioinnovation Centre, IBAB Campus, Electronic City Phase 1, Bangalore, 560100, India., Shetty P; Central Research Laboratory, KS Hegde Medical Academy (KSHEMA), NITTE Deemed to be University, Mangaluru, 575018, India.; Department of Biochemistry, KS Hegde Medical Academy (KSHEMA), NITTE Deemed to be University, Mangaluru, 575018, India., Rao RSP; Center for Bioinformatics, NITTE Deemed to be University, Mangaluru, 575018, India. drrsprao@gmail.com.
Jazyk: angličtina
Zdroj: Virus genes [Virus Genes] 2023 Jun; Vol. 59 (3), pp. 343-350. Date of Electronic Publication: 2023 Feb 06.
DOI: 10.1007/s11262-023-01975-3
Abstrakt: The recent widespread emergence of monkeypox (mpox), a rare and endemic zoonotic disease by monkeypox virus (MPXV), has made global headlines. While transmissibility (R 0 ≈ 0.58) and fatality rate (0-3%) are low, as it causes prolonged morbidity, the World Health Organization has declared monkeypox as a public health emergency of international concern. Thus, effective containment and disease management require quick and efficient detection of MPXV. In this bioinformatic overview, we summarize the numerous molecular tests available for MPXV, and discuss the diversity of genes and primers used in the polymerase chain reaction-based detection. Over 90 primer/probe sets are used for the detection of poxviruses. While hemagglutinin and A-type inclusion protein are the most common target genes, tumor necrosis factor receptor and complement binding protein genes are frequently used for distinguishing Clade I and Clade II of MPXV. Problems and possibilities in the detection of MPXV have been discussed.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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